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Prunus mume seed cultivation method

A cultivation method and seed technology, applied in the field of garden plant cultivation, can solve the problems of hard-won hybrid seeds, low germination rate, heavy workload, etc., and achieve the effects of reducing the chance of infection by diseases and insect pests, low cost and simple operation

Inactive Publication Date: 2014-05-14
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The workload of hybridization of plum blossom varieties is heavy, the seed setting rate is low, and the acquisition of hybrid seeds is hard to come by, and the low germination rate is the bottleneck problem restricting the hybrid breeding of plum blossoms

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The effect of embodiment 1 inner testa and soaking liquid on plum blossom seed dormancy and germination

[0022] Randomly select the ripe fruit of the plum blossom "Xuemei", remove the pericarp and outer testa, take out the seeds and soak them in 70% alcohol for 30 seconds, rinse with sterile distilled water twice for disinfection, and then gently peel off the inner testa with sterilized tweezers . Soak the plum blossom seeds with the inner testa peeled off in clean water and plum blossom endocarp extract for 1 day (22°C), take out the seeds, wash them once with sterile distilled water, and then place them in a culture box. Add wet sand to the culture box so that the seeds cover 1 cm of wet sand, and place them in an environment of 22°C for cultivation. After 4 days, the seeds will start to germinate. At 12 days, count the germination rate (number of germinated seeds / total number of seeds for the test) ×100%).

[0023] Control (without peeling off the inner testa): Ta...

Embodiment 2

[0032] Example 2 The effect of gibberellin concentration on the germination rate of plum blossom seeds without endocardium

[0033] According to the test method in Example 1, the plum blossom seeds stripped of the inner testa were soaked with clear water and gibberellin solutions of different concentrations respectively, and then cultivated and germinated, and the germination rate was counted in 12 days, and in 1 month, the statistics Seedling rate (number of seedlings / total number of seeds for the test × 100%), the results are shown in Table 2.

[0034] Table 2 Effect of gibberellin concentration on germination rate and seedling rate of plum blossom seeds without endocarp

[0035]

[0036] Compared with the clean water control, 250mg / L gibberellin solution can increase the germination rate of plum blossom seeds without endocardium, and when the concentration of gibberellin reached 400mg / L, the germination rate of plum blossom seeds without endocarp decreased significantly....

Embodiment 3

[0038] A method for cultivating plum blossom seeds, comprising the following steps:

[0039] Take the mature fruit of "Xuemei" as the female parent, remove the pericarp and outer testa, take out the seeds and soak them in 70% alcohol for 30 seconds, rinse twice with sterile distilled water for disinfection; then gently peel off the inner seeds with sterilized tweezers skin (membranous seed coat), do not damage the germ; soak the seeds without the endocarp in clean water at 22°C for 24 hours; take out the seeds from the clean water, rinse once with sterile distilled water, and then place them in the culture box. Add wet sand (containing 1% rice bran and 0.003% carbendazim to the weight percentage of the wet sand) into the culture box according to 5 times the seed volume, so that the seeds are covered with 1 cm of wet sand, and placed in an environment of 22 °C Cultivate in medium until the seedlings grow; when the seedlings grow to about 15cm in height, transplant the seedlings...

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Abstract

The invention discloses a prunus mume seed cultivation method which comprises the steps of seed preprocessing, dormancy breaking, sprouting cultivation, seedling transplantation and the like. According to the step of dormancy breaking, endothelia of seeds are peeled, the seeds are placed into clean water or a 100mg / L to 400mg / L gibberellin solution and soaked at the temperature of 22 DEG C for one day. According to a traditional prunus mume seed cultivation method, the prunus mume seed need to be stored at the low temperature for more than two months before cultivation so as to break the period of dormancy of the prunus mume seeds; according to the prunus mume seed cultivation method, the healthy prunus mume seedlings can be obtained within two weeks, and compared with the prior art, the cultivation is more rapid and efficient. According to the prunus mume seed cultivation method, compared with the germination rate, ranging from 30 percent to 40 percent, of an existing cultivation method, the germination rate of the prunus mume seeds can be improved to be more than 80 percent, and the germination rate is improved by one time approximately.

Description

technical field [0001] The invention belongs to the technical field of garden plant cultivation, and in particular relates to a method for cultivating plum blossom seeds. Background technique [0002] Plum blossom (Prunus mume Sieb.et Zucc) is one of the ten traditional famous flowers in China. The seeds of plum blossom have the characteristics of deep dormancy. Before cultivation, they usually need to be stored at 4°C for more than two and a half months to break the dormancy. This cultivation method takes a long time. During the long-term preservation process, the seeds are easily infected with mold and rotted, resulting in a low seedling rate. In particular, the germination rate of plum blossom seeds was not high after the above treatment, for example, the germination rate of the hybrid seeds of "Xuemei" and "Fenpi Gongfen" was only 39.1% after the above treatment. The hybridization workload of plum varieties is heavy, the seed setting rate is low, and the hybrid seeds ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01C1/00
Inventor 包满珠张金波张俊卫周忠诚
Owner HUAZHONG AGRI UNIV
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