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Method for extracting, separating and purifying isoflavone active components biochanin A and genistein from dalbergia odorifera T.Chen leaves

A technology for chickpea sprouts and genistein, which is applied in the directions of organic chemistry, fermentation, etc., can solve the problems of high separation cost and difficulty in separating chickpea sprouts A, and achieves low dosage, low separation cost, high efficiency and reasonableness. The effect of using

Inactive Publication Date: 2014-05-07
NORTHEAST FORESTRY UNIVERSITY +1
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Problems solved by technology

In terms of raw material sources, most of the traditional techniques are to extract and purify chickpein A from leguminous herbs (such as red clover, chickpea). Due to the limitation of separation conditions, purification process, raw material picking and content, etc. , the separation of chickpein A is more difficult, and the separation cost is also higher

Method used

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  • Method for extracting, separating and purifying isoflavone active components biochanin A and genistein from dalbergia odorifera T.Chen leaves
  • Method for extracting, separating and purifying isoflavone active components biochanin A and genistein from dalbergia odorifera T.Chen leaves
  • Method for extracting, separating and purifying isoflavone active components biochanin A and genistein from dalbergia odorifera T.Chen leaves

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example 1

[0021] Example 1 Extraction, Separation and Purification of Biochanin A and Genistein in Sandalwood Leaf

[0022] Take 1000g of dry Dalbergia leaves, add water, take 0.2% solid weight compound enzyme (cellulase, pectinase, glucosidase) activated compound enzyme solution, add it to the homogenate induction slurry, and continuously homogenize and break Enzyme induction 8min. After induction of homogenate crushing and enzymatic hydrolysis, the obtained solids were extracted with 70% ethanol ultrasonically for 5 times, each time for 60 minutes. Cavitation vibration dispersion for 40 minutes, static for 20 minutes, the obtained dispersion was extracted 3 times with 6.5L ethyl acetate, the extract was concentrated under reduced pressure and evaporated to dryness, the obtained solid was suspended in water, and the concentration of the liquid was configured to be 15 mg / mL of suspension drug solution, at the same time, ADS-5 macroporous adsorption resin was packed into a column by we...

example 2

[0023] Example 2 Extraction, Separation and Purification of Biochanin A and Genistein in Sandalwood Leaf

[0024]Take 3.56kg of fresh Dalbergia leaves (dry weight is 1kg), take 1.5% solid weight compound enzyme (cellulase, pectinase, glucosidase) activated compound enzyme solution and add it to the homogenate induction slurry , continuous homogenization and crushing enzyme induction for 10 min. After induction of homogenate crushing and enzymatic hydrolysis, suction filtration, the obtained solids were extracted 5 times with 95% ethanol ultrasonic assistance, each time for 60 minutes, and then the enzymatic hydrolysis liquid and ethanol extract were combined and concentrated under reduced pressure, then added 40°C water for emptying Shake and disperse for 30 minutes, stand still for 15 minutes, extract the obtained dispersion three times with ethyl acetate, concentrate the extract under reduced pressure and evaporate to dryness, suspend the obtained solid in water, and prepare...

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Abstract

The invention relates to a method for extracting, separating and purifying isoflavone active components biochanin A and genistein from dalbergia odorifera T.Chen leaves. The employed technical scheme comprises that biochanin A and genistein are respectively obtained both with the purity of 95% or more by taking dalbergia odorifera T.Chen leaves as raw materials and employing homogenate fragmentation enzyme induction technology, solvent extraction technology, cavitation oscillation dispersion technology, macroporous adsorption resin enrichment technology, normal-phase silica gel medium-pressure column chromatography separating technology, low-temperature crystallization and recrystallization technology, and the like. Because dalbergia odorifera T.Chen leaves are abundant in resource, the extraction process is simple, the separation cost is low and the method is time-saving and labor-saving, the method provided by the invention is applicable to large-scale production in industry, and has extremely high medical science value and wide market prospect.

Description

technical field [0001] The invention relates to a method for extracting, separating and purifying the active components of Chinese herbal medicine biochanin A and genistein from the leaves of Sandalwood lanceolata, belonging to the technical field of separation and purification of natural medicines. Background technique [0002] Dalbergia odorifera T.Chen, also known as rosewood, belongs to the genus Dalbergia of the family Fabaceae. The medicinally active part is the dry heartwood of its trunk and root, which has the effects of promoting qi and blood circulation, relieving pain and stopping bleeding, and is often used for abdominal pain, liver depression and hypochondriac pain, bruises, traumatic bleeding, anti-inflammatory and anticoagulant It can also be used in combination with other blood-activating and stasis-removing drugs for the treatment of cardiovascular diseases. At present, the planting of woody plants such as sandalwood is relatively extensive, while the resea...

Claims

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Application Information

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IPC IPC(8): C12P17/06C07D311/36C07D311/40
Inventor 付玉杰王微马飞跃罗猛顾成波李吉魏作富
Owner NORTHEAST FORESTRY UNIVERSITY
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