Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Alleles Regulating Two Main Stem Cuticle Hair Types of Upland Cotton and SSCP Molecular Markers for Their Identification

An allele and upland cotton technology, applied in genetic engineering, plant genetic improvement, microbial measurement/inspection, etc., can solve problems such as the influence and function of GhHD-1 gene that have not been explained

Inactive Publication Date: 2016-05-18
ZHEJIANG FORESTRY UNIVERSITY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned studies did not explain the influence and effect of GhHD-1 gene on stem epidermis or fiber quality.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Alleles Regulating Two Main Stem Cuticle Hair Types of Upland Cotton and SSCP Molecular Markers for Their Identification
  • Alleles Regulating Two Main Stem Cuticle Hair Types of Upland Cotton and SSCP Molecular Markers for Their Identification
  • Alleles Regulating Two Main Stem Cuticle Hair Types of Upland Cotton and SSCP Molecular Markers for Their Identification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] The technical solutions of the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0025] 1. DNA sequence differences of GhHD1 gene in different upland cotton

[0026] Our laboratory designed specific primers for the HD1 gene (At-GhHD1) of the upland cotton A genome, and used the primers to amplify the full-length sequence of the GhHD1 gene from different (50) upland cotton varieties. Sequencing analysis showed that there were two types of The DNA sequence is represented by type A1 and type A2. Sequence comparison shows that there are 8 base differences between type A1 and type A2, 5 of which are in the exon (Appendix 1), making them have 5 amino acid differences ( figure 1). Analysis of expression in different tissues showed that GhHD1 gene was predominantly expressed in stems.

[0027] 2. Observation and analysis of upland cotton stalk epidermis

[0028] The phenotype of the stem epi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses alleles for regulating and controlling two types of main stem table fur types of upland cotton, and a single stranded conformational polymorphism (SSCP) molecular marker for identification thereof. On the research basis of defining that an HDI gene in a homeobox-homeodomain-leucine zipper protein family IV is a key gene of affecting table fur generation of the upland cotton, the alleles GhHD1a and b for regulating and controlling regeneration of two types of stem table furs of the upland cotton are identified for the first time; a molecular marker for screening the two alleles by using the SSCP technology is invented. The molecular marker can rapidly identify two kinds of table fur types. The alleles prove that two genetypes of the HD1 have close relationship to two table fur types, a foundation is established for researching the molecular mechanism of generating table furs and fibers, the deoxyribonucleic acid (DNA) molecular marker capable of directly identifying the table fur type of the upland cotton in a laboratory is developed, and a rapid technology is provided for rapidly breeding different fur types of new cotton varieties.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an allele that regulates two types of main stalk hair types of upland cotton and an SSCP molecular marker used for its identification. Background technique [0002] Fiber is the main product harvested in cotton production and is the main raw material for the textile industry. High-yield and high-quality fiber is the ultimate goal of cotton breeding. Cotton fiber can be divided into two categories: fuzzy fiber (<5mm) and lint fiber (lint fiber) according to the length. The linter fibers are not only short, but also tightly attached to the surface of the seed. Lint fibers can be divided into short fibers (<21mm), medium and long fibers (28-32mm) and long fibers (>35mm). Cotton fiber is protruding and extending from the epidermal cells of the seed. Its growth and development can be artificially divided into four stages: the initiation of fiber (flowering to 5 days after flower...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12Q1/68
Inventor 戎均康何沙娥丁明全李飞飞周伟曹跃芬
Owner ZHEJIANG FORESTRY UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com