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Method for induced mutation breeding by hypergravity

A technology of mutation breeding and supergravity, which is applied in the field of breeding, can solve the problems of small variation range, slow speed, and long cycle, and achieve the effects of increased efficiency and variation range, simple breeding, and short cycle

Inactive Publication Date: 2014-04-30
李旭业
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims to solve the problems of long cycle, slow speed, low efficiency and small variation range in the existing crop and forage breeding, thereby providing a method of hypergravity induced breeding

Method used

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Examples

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specific Embodiment approach 1

[0015] The specific embodiment one, a kind of method for hypergravity mutation breeding, it is realized by the following steps:

[0016] Step 1, put the seeds or germinated seeds into a centrifuge, and carry out hypergravity mutagenesis under the condition that the supergravity range is 200g-5000g, and the hypergravity mutagenesis time is 1 hour to 48 hours; make the seeds or germinated seeds in Genetic mutations occur under high-gravity environmental conditions;

[0017] Step 2, using the seeds or germinated seeds without hypergravity mutagenesis as a standard, screening the seeds or germinated seeds after hypergravity mutagenesis in step 1 to obtain a mutagenic strain; and using the obtained mutagenic strain Select and breed 3 to 4 generations to obtain genetically stable germplasm resources, and breed inbred lines or varieties with uniform traits, high combining ability, excellent agronomic traits, and high yield (excellent and stable);

[0018] inbred lines or varieties; ...

specific Embodiment approach 2

[0020] Specific embodiment two, this specific embodiment is the further limitation of the method for a kind of hypergravity mutation breeding described in specific embodiment one, before putting seed or germinated seed into centrifuge, at first adopt to seed or germinated seed Wash with sterile water.

specific Embodiment approach 3

[0021] Specific embodiment three, this specific embodiment is the further limitation of the method for a kind of hypergravity mutation breeding described in specific embodiment one, in step one, adopt cotton or gauze to put into centrifuge seed or germinate seed to protect .

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Abstract

The invention relates to a breeding method, and particularly relates to a method for induced mutation breeding by hypergravity. The invention aims to solve the problem that the existing breeding of crops and pasture plants has long period, low speed, low efficiency and low mutation. The method comprises the steps of placing seeds or germinated seeds into a centrifuge, performing hypergravity induced mutation under hypergravity range of 200-5,000g for 1-48h; screening the seeds or the germinated seeds subjected to hypergravity induced mutation to obtain a mutant strain; breeding the obtained mutant strain for 3-4 generations to obtain germplasm resource with genetic stability, and breeding to obtain a good and stable self-bred line or variety; and performing cross breeding on the obtained good and stable self-bred line or variety, and selecting good germplasm resource to prepare cross combination as parent plant to complete induced mutation breeding by hypergravity. The breeding method provided by the invention is suitable for breeding new varieties of crops and pasture plants.

Description

technical field [0001] The present invention relates to a breeding method. Background technique [0002] In the process of breeding new varieties of crops and forages, it is often difficult to achieve breakthroughs due to the limitation of germplasm resources. Conventional crops and forages have a long cycle, slow speed, low efficiency, and small variation. Contents of the invention [0003] The present invention aims to solve the problems of long cycle, slow speed, low efficiency and small variation range in existing crop and forage breeding, thereby providing a method for hypergravity induced breeding. [0004] A method for hypergravity mutation breeding, which is realized by the following steps: [0005] Step 1, put the seeds or germinated seeds into a centrifuge, and carry out hypergravity mutagenesis under the condition that the supergravity range is 200g-5000g, and the hypergravity mutagenesis time is 1 hour to 48 hours; make the seeds or germinated seeds in Geneti...

Claims

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Application Information

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IPC IPC(8): A01H1/06A01H1/04A01H1/02
Inventor 李旭业董扬王洪宝杨晓杰徐艳霞张超于侃超
Owner 李旭业
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