Method for pyrolysis of thymosin beta-4
A technology of thymosin and lysate, which is applied in the field of polypeptide cleavage, can solve the problems of unsuitable thymosin β4, etc., and achieve the effect of improving the purity of crude peptide, reducing production cost, and increasing the total yield
Active Publication Date: 2014-04-16
哈尔滨吉象隆生物技术有限公司
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[0012] Due to the particularity of thymosin β4, the above method is not suitable for the cleavage of thymosin β4. After research, the present invention has found a new cleavage method, which is especially suitable for the cleavage of thymosin β4.
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Abstract
The invention discloses a method for pyrolysis of thymosin beta-4. The method comprises the step of carrying out pyrolysis on thymosin beta-4 peptide resin to obtain crude peptide, wherein a mixed solvent of trifluoroacetic acid, thioanisole, dithioglycol, phenol, water, dimethyl sulfide and ammonium iodide is used as a pyrolysis reagent in the pyrolysis process. According to the method for pyrolysis of thymosin beta-4, the purity of crude peptide is greatly improved, meanwhile the total yield is greatly increased, the production cost is reduced, and the method is simple in process and convenient for industrial production.
Description
technical field [0001] The invention relates to a method for cleavage of polypeptides, in particular to a method for cleavage of thymosin β4. Background technique [0002] Thymosin was first extracted from calf thymus by Goldstein and White in 1966. According to the different isoelectric points, thymosin can be divided into 3 types: α (PI<5), β (5<PI<7) and γ (PI>7). Constructs have been identified, and there are three main types in the human body, namely thymosin β4 (Tβ4), Tβ10 and Tβ15, of which thymosin β4 has the highest content. Thymosin β4 is a highly conserved water-soluble polypeptide composed of 43 amino acid residues, with a relative molecular mass of 4964. [0003] The molecular formula of thymosin β4 is: [0004] Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu -Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser-OH Thymosin β4 is considered to be predominantly spherical Ac...
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IPC IPC(8): C07K14/575C07K1/04
CPCC07K14/57581
Inventor 冷国庆余荣熹王艳王浩迟帅刘宇帅田辉张琪李迎新肖明辉李喜全赵民喜苏宏健
Owner 哈尔滨吉象隆生物技术有限公司
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