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Ammopiptanthus mongolicus drought induced related gene, and expression vector and application thereof

A technology of drought induction and expression vector, which is applied in the fields of application, genetic engineering, plant gene improvement, etc., and can solve the problem that it is difficult to fully reflect the comprehensive ability of plants

Active Publication Date: 2015-05-06
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the identification of a single drought resistance index used in traditional drought resistance research is difficult to fully reflect the comprehensive ability of plants to adapt to drought. Only the comprehensive evaluation of multiple indicators can accurately reflect the true level of drought resistance of plants.

Method used

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  • Ammopiptanthus mongolicus drought induced related gene, and expression vector and application thereof
  • Ammopiptanthus mongolicus drought induced related gene, and expression vector and application thereof
  • Ammopiptanthus mongolicus drought induced related gene, and expression vector and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1 Cloning of Drought-Induced Expression Genes

[0061] 1. Test material

[0062] 1.1 Enzymes and kits: cDNA Synthesis kits were purchased from Takara and Toyobo companies; La Taq and Ex Taq enzymes were purchased from Treasure Bioengineering (Dalian) Co., Ltd.; RACE kits were purchased from Invitrogen Life Technologies, USA; gel recovery kits were purchased from Biomiga; the sequencing carrier T1-easy was purchased from Beijing Quanshijin Biotechnology Co., Ltd.

[0063] 1.2 Primers and sequencing

[0064] 3’RACE adapter TACCGTCGGTCCACTAGTGAGTTCACTATAGG(T)20

[0065] OP TACCGTCGGTCCACTAGTGAGTT

[0066] IP CCGTCGGTCCACTAGTGAGTTCACTATAGG

[0067] 3' specific primer OP GGAATGAAGTTGAGGTACCAA

[0068] IP ATGTTAATGCCTCTGATGGGGTT

[0069] 5'RACE5'OP GACTGGAGCACGAGGACACTG

[0070] 5'IP GAGGACACTGACATGGACTGAAGGAGTAG

[0071] 5'-specific primer 5'OP CATCAGAGGCATTAACATGAGG

[0072] 5'IP TTGGAGCATGAAGA...

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Abstract

The invention discloses an ammopiptanthus mongolicus drought induced related gene, and an expression vector and application thereof, and belongs to the fields of separation and application of plant resistance genes. The invention provides a drought induced gene cDNA (complementary deoxyribonucleic acid) full-length sequence separated from ammopiptanthus mongolicus. A nucleotide sequence is shown as SEQ ID NO.1, and a coded amino acid sequence is shown as SEQ ID NO.2. The invention also provides a recombinant plant expression vector and a recombinant host cell containing the gene. The invention further provides a method for improving the drought stress resistance of a plant or cultivating a new variety of a drought-resistant transgenic plant. The method comprises the following steps: constructing the recombinant plant expression vector containing the drought induced related gene cDNA; transforming the recombinant plant expression vector into a receiver plant or a plant cell; performing cultivation and screening to obtain a transgenic plant with improved drought stress resistance. The separated drought induced related gene plays an important application role in improvement of the plant adverse resistance or cultivation of the new variety of a transgenic plant with the improved drought stress resistance.

Description

technical field [0001] The present invention relates to a resistance gene related to environmental stress isolated from plants, in particular to a drought-induced related gene isolated from A. mongolicus. The invention relates to the application in breeding new varieties of drought-resistant transgenic plants, belonging to the field of separation and application of plant resistance genes. Background technique [0002] Drought is one of the important adversity factors that affect the normal growth and development of plants. It can affect a series of changes in plant physiology, morphology, biochemistry and molecular structure, and then affect plant growth, development and yield. The main manifestations are: plant cell active oxygen content Increase, change of composition and content of osmotic adjustment substances, decrease of plant cell water potential, increase of biomembrane system destruction and permeability, and cause disorder of membrane enzyme activity. In addition,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/00
Inventor 郭惠明程红梅孙朋卫
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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