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Method for controlling injurious insect

A technology for pests, oriental armyworms, applied in the fields of botanical equipment and methods, biochemical equipment and methods, pesticides, etc.

Active Publication Date: 2014-04-02
BEIJING DABEINONG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Plants transgenic for Cry1A gene have been proved to be resistant to Lepidoptera pests such as corn borer, cotton bollworm, and fall armyworm. to report

Method used

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  • Method for controlling injurious insect
  • Method for controlling injurious insect
  • Method for controlling injurious insect

Examples

Experimental program
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Effect test

no. 1 example

[0088] The first embodiment, the acquisition and synthesis of Cry1A gene

[0089] 1. Obtain the Cry1A nucleotide sequence

[0090] The amino acid sequence (818 amino acids) of the Cry1Ab-01 insecticidal protein, as shown in SEQ ID NO: 1 in the sequence listing; the Cry1Ab-01 core encoding the amino acid sequence (818 amino acids) corresponding to the Cry1Ab-01 insecticidal protein Nucleotide sequence (2457 nucleotides), as shown in SEQ ID NO:4 in the sequence listing. The amino acid sequence (615 amino acids) of the Cry1Ab-02 insecticidal protein, as shown in SEQ ID NO: 2 in the sequence listing; Cry1Ab-02 encoding the amino acid sequence (615 amino acids) corresponding to the Cry1Ab-02 insecticidal protein Nucleotide sequence (1848 nucleotides), as shown in SEQ ID NO:5 in the sequence listing.

[0091] The amino acid sequence (1177 amino acids) of the Cry1A.105 insecticidal protein, as shown in SEQ ID NO: 3 in the sequence listing; Cry1A.105 encoding the amino acid sequence...

no. 2 example

[0098] The second embodiment, construction of recombinant expression vector and transformation of recombinant expression vector into Agrobacterium

[0099] 1. Construction of a recombinant cloning vector containing the Cry1A gene

[0100] The synthesized Cry1Ab-01 nucleotide sequence was connected to the cloning vector pGEM-T (Promega, Madison, USA, CAT: A3600), and the operation steps were carried out according to the instructions of the pGEM-T vector produced by Promega Company to obtain the recombinant cloning vector DBN01-T , its construction process is as follows figure 1 Shown (wherein, Amp represents the ampicillin resistance gene; f1 represents the replication origin of phage f1; LacZ is the LacZ start codon; SP6 is the promoter of SP6 RNA polymerase; T7 is the promoter of T7 RNA polymerase; Cry1Ab-01 is the promoter of Cry1Ab -01 nucleotide sequence (SEQ ID NO: 4); MCS is a multiple cloning site).

[0101] Then, the recombinant cloning vector DBN01-T was transformed...

no. 3 example

[0118] The third embodiment, the acquisition and verification of corn plants transferred to the Cry1A gene

[0119] 1. Obtaining corn plants transferred to the Cry1A gene

[0120] According to the commonly used Agrobacterium infection method, the immature embryos of the aseptically cultured maize variety Zong 31 (Z31) were co-cultured with the Agrobacterium described in 3 in the second example, so that the T-DNA in recombinant expression vectors DBN100124, DBN100053, DBN100003, DBN100075, DBN100029 and DBN100076 (including the promoter sequence of corn Ubiquitin gene, Cry1Ab-01 nucleotide sequence, Cry1Ab-02 nucleotide sequence, Cry1A.105 nucleotide sequence Acid sequence, Vip3Aa nucleotide sequence, Cry1Fa nucleotide sequence, Cry2Ab nucleotide sequence, PMI gene and Nos terminator sequence) were transferred into the maize genome, and the maize with Cry1Ab-01 nucleotide sequence was obtained Plants, corn plants transferred to Cry1Ab-02 nucleotide sequence, corn plants transf...

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Abstract

The invention relates to a method for controlling eastern mythimna separata injurious insects. The method comprises the step as follows: the eastern mythimna separata injurious insects contact Cry1A protein. The eastern mythimna separata injurious insects are controlled by producing the Cry1A protein capable of killing the eastern mythimna separate inside a plant body. Compared with an agricultural prevention method, a chemical prevention method and a physical prevention method used in the prior art, the whole growth period and whole plant protection is carried out on the plant, so as to prevent the damage of the eastern mythimna separata injurious insects. The method is free of pollution, free of residue, stable and thorough in effect, simple, convenient and economical.

Description

technical field [0001] The invention relates to a method for controlling pests, in particular to a method for controlling oriental armyworm damage to plants by using Cry1A protein expressed in plants. Background technique [0002] Oriental Armyworm (Mythimna seperata) belongs to Lepidoptera Noctuidae. It is an omnivorous, migratory, and intermittently explosive pest that mainly damages wheat, corn, sorghum, rice and other food crops and pastures. It has been eradicated in my country. Except for Xinjiang, which has not yet occurred, there are hazards in other places. Eastern armyworm likes to eat leaves, and after the third instar, it can bite the whole leaf into a notch shape, or eat all the heart leaves to form heartless seedlings. When the five to six instars reach the overeating period, they can eat up all the above-ground parts of the seedlings, or eat up the entire leaves of the plants, leaving only the veins, resulting in severe yield reduction, or even no harvest. In ...

Claims

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Application Information

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IPC IPC(8): A01N47/44A01N57/16A01P7/04C12N15/84A01H5/00A01N63/50
CPCC12N15/8285Y02A40/146A01N63/50A01N63/23
Inventor 李胜兵王利君刘敬庞洁韩超岳昌锋
Owner BEIJING DABEINONG BIOTECHNOLOGY CO LTD
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