Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Rat model with growth hormone deficiency after cerebral trauma

A growth hormone deficiency and rat model technology, applied in veterinary surgery, medical science, veterinary instruments, etc., can solve the problems of poor repeatability, growth hormone deficiency, low success rate, etc., and achieve a large-scale deployment Effect

Inactive Publication Date: 2014-03-12
首都医科大学康复医学院
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the success rate of traditional modeling methods is low and the reproducibility is poor. Many traumatic brain injury rats did not cause growth hormone deficiency

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Rats were fixed on a Kopf stereotaxic apparatus, and anesthesia was maintained throughout the surgical procedure. Then the hair on the top of the rat's head was shaved, and the operation area was disinfected in the order of 75% alcohol, iodophor, and 75% alcohol. A 3cm long incision was made along the midline of the head, the bilateral scalp was fixed, the periosteum was peeled off, and the bilateral frontal lobes were exposed. The bilateral frontal lobe craniotomy was performed using a special drill for dentists. Perform bone flap surgery at a distance of 3 mm from the midline between the bregma and the herringbone suture. Be careful not to damage the dura mater, and the diameter of the bone flap must be larger than the diameter of the percussion device head (5 mm) to ensure that the brain tissue will not touch the surrounding bone. organize. Controlled cortical percussion surgery is performed with a CCI percussion device. The percussion parameters are a. percussion s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a rat model with growth hormone deficiency after cerebral trauma. The model is manufactured by virtue of the following steps: fixing and narcotizing a rat; shaving off hair in the top of a rat head; sterilizing the operation area; making a 3cm long cut along the median line of the head, fixing bilateral scalps, peeling periosteums, exposing bilateral frontal lobes, and performing a bilateral frontal lobes craniotomy by using a drill. Craniotomy is performed between the bregmatic fontanel and the lambdoidal suture and 3cm from the central line, endocranium cannot be injured, the diameter of a bone flap can be 5mm more than the diameter of the head part of an impactor; a controlled cortical impact operation is performed by utilizing a CCI (chronic constriction injury) impactor, wherein impact parameters include the impact speed of 3.5m / s, the impact depth of 1.5mm, and the stay time of 400m sec. The bone flaps are not returned, and the head cut is sutured after the operation.

Description

technical field [0001] The invention relates to the production of an animal model, in particular to a growth hormone-deficient rat model of traumatic brain injury. Background technique [0002] Growth hormone deficiency after traumatic brain injury may lead to slow recovery of neurological function and even cognitive decline. In recent years, there have been many subjects in growth hormone deficiency experiments using traumatic brain injury rats. However, the traditional modeling method has a low success rate and poor reproducibility, and many traumatic brain injury rats did not cause growth hormone deficiency. [0003] In the present invention, on the basis of the rat model of traumatic brain injury, after adjusting the experimental parameters and the impact site, the rate of growth hormone deficiency in rats with traumatic brain injury is as high as 90%, and the degree of reduction is obvious. Contents of the invention [0004] The invention provides a growth hormone-d...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61D1/00
Inventor 张皓凌锋
Owner 首都医科大学康复医学院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com