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Method for extracting brown algae polyphenols

A brown algae polyphenol and extraction method technology, applied in the field of marine organisms, can solve the problems of unfavorable industrial production, low product safety, high alkaloid content, and achieve the advantages of industrial production, high yield, high product yield and purity. Effect

Inactive Publication Date: 2014-03-05
WEIHAI YUWANG GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the products obtained by these methods have the disadvantages of high content of caffeine and a

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The extraction of brown algae polyphenols is carried out through the following steps:

[0026] Step 1: Weigh 1000g of fresh kelp, remove sediment, phytoplankton and other attachments, then rinse it with tap water repeatedly, dry it in an oven at 105°C to constant weight, and obtain a solid of about 100g, mash it with tissue Pulp machine broken;

[0027] Step 2: Add 0.002% tert-butyl-p-hydroxyanisole (BHA) to the crushed biological raw material according to its wet weight, mix it with an aqueous solution containing 70% ethanol at a ratio of 1:2, stir thoroughly and place it in ultrasonic treatment 3min, placed in a constant temperature water bath at 60°C, oscillated, and soaked in the dark for 1 hour, separated the supernatant, and distilled off the organic solvent under reduced pressure;

[0028] The third step: place it in a freezer at 4°C for 8 hours, separate the supernatant, wash the supernatant with a 1:1 chloroform aqueous solution, and distill under reduced pres...

Embodiment 2

[0033] The extraction of brown algae polyphenols is carried out through the following steps:

[0034] Step 1: Weigh 1000g of fresh sea millet, remove sediment, phytoplankton and other attachments, then rinse with tap water repeatedly, dry in an oven at 105°C until constant weight, obtain about 50g of solid matter, pound with tissue crushing homogenizer crushing;

[0035] The second step: add 0.02% 2,6-di-tert-butylated hydroxytoluene (BHT) to the wet weight of the crushed biological raw material, mix it with an aqueous solution containing 80% acetone at a ratio of 1:4, and stir thoroughly Afterwards, put it in ultrasonic treatment for 6 minutes, put it in a constant temperature water bath at 40°C, oscillate, and leaching in the dark for 2 hours, separate the supernatant, and remove the organic solvent by distillation under reduced pressure;

[0036] The third step: place it in a freezer at 2°C for 6 hours, separate the supernatant, wash the supernatant with a petroleum ether ...

Embodiment 3

[0041] The extraction of brown algae polyphenols is carried out through the following steps:

[0042] Step 1: Weigh 1000g of fresh sargassum, remove the sediment, phytoplankton and other attachments, then rinse it repeatedly with tap water, dry it in an oven at 105°C to constant weight, and obtain a solid of about 50g, pound it with tissue crushing homogenizer crushing;

[0043] Second step: Add 0.004% 2,6-di-tert-butylated hydroxytoluene (BHT) and 0.004% sodium sulfite to the broken biological raw material by wet weight, and mix with an aqueous solution containing 50% methanol and 25% toluene by 1 : 3, mixed in a ratio of 3, fully stirred, placed in ultrasonic treatment for 4 minutes, placed in a 50°C constant temperature water bath, oscillated, and leached in the dark for 3 hours, separated from the supernatant, and removed the organic solvent by distillation under reduced pressure;

[0044] The 3rd step: be placed in 3 ℃ of refrigerators and stand still for 6 hours, separa...

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Abstract

The invention provides a method for extracting brown algae polyphenols from organisms. The brown algae polyphenols are extracted through the following steps: raw material pretreatment; leaching; cooling and washing; resin adsorption; eluting; and freeze drying. According to the technical scheme, the method has the advantages of high yield, low cost, convenience for industrial production and the like.

Description

technical field [0001] The invention relates to the field of marine organisms, in particular to a method for extracting brown algae polyphenols from brown algae. Background technique [0002] Brown algae polyphenols are widely found in marine plant brown algae, which use glucinol as a structural unit, and are mainly divided into oligomeric phloroglucinol and Eckol polyphenols. Studies have proved that brown algae polyphenols have a wide range of biological activities such as anti-cardiovascular disease, anti-bacterial, anti-virus, algae-dissolving, anti-oxidation, anti-diabetic syndrome, liver protection and inhibition of lysinase hyaluronidase. Brown algae polyphenols have different structures and activities from land plant polyphenols, and are a unique class of natural substances. [0003] The currently reported methods for extracting brown algae polyphenols mainly use ethanol solution at room temperature to avoid light to obtain a crude extract, and then undergo steps su...

Claims

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Application Information

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IPC IPC(8): A61K36/02A61K36/03A61K47/08A61K47/02
Inventor 苗强
Owner WEIHAI YUWANG GROUP
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