Compound microorganism bacterium agent for banana stem and leaf silage and preparation method of compound microorganism bacterium agent
A technology of banana stems and leaves and compound bacterial agents, which is applied in animal feed, animal feed, applications, etc., can solve the problems of low crude protein, low degradation rate, and restricted development of banana stems and leaves, and achieve the reduction of cellulose content and huge The effect of economic benefits
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[0033] 1. Production of microbial compound bacteria agent
[0034] 1.1 Preparation of Lactobacillus plantarum freeze-dried inoculum
[0035] Lactobacillus plantarum NL4-10 was inoculated in MRS medium at 1% inoculum amount, and cultured at 37°C for 36 hours to a stable stage. Centrifuge at 10,000×g for 10 minutes at 4°C, remove the supernatant, wash the bacteria sludge twice with sterile phosphate buffer saline with a concentration of 60 mM and a pH value of 6.5, then centrifuge at 10,000×g for 10 minutes, and resuspend Add an equal volume of 10% (m / v) skim milk solution to 200 mL of sterile phosphate buffer as a protective agent, pre-freeze at -20°C for 2 hours, then pre-freeze at -80°C for 8 hours, and pre-freeze Then put it into a freeze dryer for freeze-drying, the cold trap temperature is -60°C, the vacuum degree is 4-6Pa, and the vacuum freeze-drying time is 16 hours, so that the Lactobacillus plantarum freeze-dried microbial agent can be obtained.
[0036] Among them,...
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