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Production method of bletilla seed stems

A production method and Bletilla striata technology, applied in the biological field, can solve problems such as low survival rate of direct seedling cultivation, and achieve the effect of overcoming the low survival rate of direct cultivation and solving the problem of provenance.

Inactive Publication Date: 2014-02-19
贵州省生物技术研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is: provide a kind of production method of bletilla striata seed stem, it has overcome the problem of the low survival rate of bletilla striata tissue culture seedling directly hardening seedling, has solved the provenance problem of bletilla striata artificial large-scale planting, for the sustainable development of bletilla striata and Lay the foundation for development and utilization

Method used

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Embodiment Construction

[0016] Embodiments of the present invention: the production method of bletilla striata stem, comprises the steps:

[0017] 1) Treatment and inoculation of explants: Artificial pollination is carried out during the flowering season of Bletilla striata each year, Bletilla striata capsules are collected in September-October, the surface dust is washed with water, and after natural drying, the mass percentage concentration of Soak in 75% alcohol for 30 s, then soak in 0.1% mercuric solution for 8 min, then wash with sterile water for 5 times, then use sterile filter paper to dry the water on the surface of Bletilla striata capsule, and dissect it with sterile Cut open the capsule longitudinally with a knife, take out the seeds in the Bletilla striata capsule, spread them evenly on the surface of the seed germination medium, and cultivate them in the cultivation room, the cultivation temperature is 25±2°C, the light intensity is 1500-2000Lx, and the light time is 12h / d; the main b...

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Abstract

The invention discloses a production method of bletilla seed stems, which comprises the following steps: 1) explant treatment and inoculating, 2) protocorm obtaining and multiplication, 3) differentiation and seedling forming of protocorms, 4) test-tube corm obtaining, 5) primary seed stem obtaining and 6) secondary seed stem obtaining. According to the method, mature bletilla seeds are subjected to aseptic seeding and culture, many test-tube seed stems are obtained, and the larger production seed stems capable of being grown in a large field are obtained by primary and secondary seed stem reproduction. The method solves the problem of a low survival rate of direct exercising of tissue culture seedlings, provides a seed source for manual massive bletilla planting, and lays a foundation for sustainable development, exploitation and utilization of bletilla.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for producing bletilla striata stems. Background technique [0002] Bletilla striata (Thunb.) Reichb.f.] is a perennial herbaceous plant of the genus Bletilla striata in the family Orchidaceae. Slightly cold in nature, bitter, sweet, and astringent, it belongs to the lung, stomach, and liver meridian, has the functions of nourishing the lung and stopping bleeding, reducing swelling and promoting muscle growth, etc. It can be used externally to treat traumatic bleeding, burns, and chapped hands and feet; in addition, Bletilla striata contains high content of Bletilla striata gum, which can be used as a suspending agent and emulsifier, and is widely used in the fields of food and chemical industry; , Textile printing and dyeing, special coatings and daily chemicals are also widely used. Bletilla striata is gorgeous in color and can be used as a beautiful ornamental plant. Ble...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 毛堂芬陈娅娅王沁刘作易
Owner 贵州省生物技术研究所
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