Method and kit for detecting oral cancer by micro nucleic acid-196a molecule and micro nucleic acid-196b molecule, and primer and probe for method and kit

Abstract

The invention discloses a method and a kit for detecting oral cancer by a micro nucleic acid-196a (miR-196a) molecule and a micro nucleic acid-196b (miR-196b) molecule, and a special primer and probe for the method and the kit. The method comprises the step of detecting expression quantities of the miR-196a molecule and the miR-196b molecule in biological specimens such as tumor tissues, blood plasma, serum, saliva or mouth wash and the like of a potential patient, wherein the miR-196a molecule has a nucleotide sequence represented by SEQ ID NO: 1, the miR-196b molecule has a nucleotide sequence represented by SEQ ID NO: 2, and if the expression quantity of the miR-196a molecule or miR-196b molecule is abnormally higher than normal value (the normal value of the miR-196a is 1.817+ / -0.38, and the normal value of the miR-196b is 7.683+ / -1.88), the condition can be concluded that the potential patient suffers from the oral cancer. The reverse transcription real-time fluorescence quantitative polymerase chain reaction technology (RT-qPCR) is used for detecting the expression quantities of the miR-196a molecule and the miR-196b molecule in the blood plasma of the patient suffering from oral cancer and normal people, the test discovers that the expression quantity of whether the miR-196a molecule or the miR-196b molecule of the patient has obvious high expression (p is less than 0.0001) in comparison with that of the normal people, the detection sensitiveness achieves 90%, and the specificity achieves 85%; the above result shows that good identification degree is achieved when the miR-196a molecule and the miR-196b molecule are applied to detecting the oral cancer.

Description

technical field [0001] The present invention provides a method for using micronucleic acid-196a (miR-196a) molecules and micronucleic acid-196b (miR-196b) molecules to detect oral cancer, especially using miR-196a molecules and miR-196b molecules as oral cancer The method of analyzing the expression levels of miR-196a molecules and miR-196b molecules in biological samples of potential patients to infer that the potential patients suffer from oral cancer. Background technique [0002] Hematologic tumor markers have value in disease detection, prognosis and monitoring treatment outcomes. Therefore, a clinically applicable blood-type tumor marker could provide better disease control. The current blood tumor markers include CEA to detect colorectal cancer, AFP to detect liver cancer, and PSA to detect prostate cancer. It has also been reported in the past that SCC antigen and Cyfra21 can be used as detection markers for head and neck cancer, but so far There are clinically use...

AI Technical Summary

Problems solved by technology

In recent years, studies have shown that many different cancers have their specific micronucleic acid expression, but the research shows that there are not many common results, indicating that the complexity of micronucleic acid and its mechanism are not really understood

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