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A process for the manufacture of products from cruciferous crops

A technology of cruciferous products, applied in the fields of application, food science, fat production, etc., which can solve the problems of not explicitly mentioning myrosinase, myrosinase-glucosinolate problem not being clearly solved, etc.

Active Publication Date: 2014-01-22
UNIVERSITY OF COPENHAGEN +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] WO2008 / 144939 does not explicitly mention myrosinase, and the myrosinase-glucosinolate problem is therefore not explicitly addressed

Method used

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  • A process for the manufacture of products from cruciferous crops
  • A process for the manufacture of products from cruciferous crops
  • A process for the manufacture of products from cruciferous crops

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0093] Example 1 - Recovery of myrosinase activity after low pH treatment

[0094]0.5 grams of Brassica napus (DM8%) full fat seeds were ground in a coffee mill and then mixed with 10.00 mL of citrate buffer varying from 1 to 200 mM. Separately, samples containing 100 mM citrate buffer and 10 mM sulfuric acid or 25 mM citrate buffer and 50 mM sulfuric acid were prepared. Mixing of ground seed material with these different buffers was performed with wet milling using an Ultra Turrax for 2 minutes. The temperature was maintained at 20°C.

[0095] Centrifuge at 4000 x g for 5 minutes to generate a supernatant and a sediment phase. Measure pH in all solutions. Measurements of UV absorbance were also performed at both 280 nm and 325 nm.

[0096] Myrosinase activity after treatment at low pH was measured using spectrophotometry, where myrosinin (1.2 mM, 500 μL) was used as the substrate, ascorbic acid was used as the activator of the reaction (10 mM, 75 μL), and phosphate buffer...

example 2

[0099] Example 2 - Glucosinolate levels in solution affected by the pH of the solution

[0100] 0.5 grams of Brassica napus (DM8%) full fat seeds were ground in a coffee mill and then mixed with 10.00 mL of citrate buffer varying from 1 to 200 mM. Separately, samples containing 100 mM citrate buffer and 10 mM sulfuric acid or 25 mM citrate buffer and 50 mM sulfuric acid were prepared. In order to achieve lower pH values, mixtures with increasing concentrations of sulfuric acid were also prepared. The sulfuric acid of these mixtures ranged from 40 mM to 150 mM. Mixing of ground seed material with these different buffers was performed with wet milling using an Ultra Turrax for 2 minutes. The temperature was maintained at 20°C.

[0101] Centrifuge at 4000 x g for 5 minutes to generate a supernatant and a sediment phase. Measure pH in all solutions.

[0102] The supernatant (1 mL) was added to an anion exchange column (1 mL Sephadex DEAE, A-25, GE Healthcare) to adsorb glucos...

example 3

[0105] Example 3 - Myrosinase activity in different pH and different buffer systems

[0106] 0.5 g of full fat seeds of Brassica napus (DM8%) were ground in a coffee mill and then mixed with 10.00 mL of buffer adjusted to range from pH 7 to pH 2 using 50 mM citrate or acetate different pH values. Mixing of ground seed material with these different buffers was performed with wet milling using an Ultra Turrax for 2 min. The temperature was maintained at 20°C.

[0107] Centrifugation was performed at 4000 xg for 5 minutes to generate a supernatant and a sediment phase. Measure pH in all solutions.

[0108] Myrosinase activity was measured using spectrophotometry, in which myrosinin (1.2 mM, 500 μL) was used as the substrate, ascorbic acid was used as the activator of the reaction (10 mM, 75 μL), and buffer (citrate or Acetate) (50 mM, variable volume, variable pH according to sample conditions) was used to keep the pH constant, and the final supernatant (variable volume, typi...

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Abstract

The present invention relates to a process for the manufacture of a product from seeds from a plant of the order Capparales comprising the steps of: providing the seeds having a water content of about 9 % by weight or less; - disrupting the seeds; - mixing the disrupted seeds with aqueous acid to provide a pH of the mixture in the range of about 1.0 to about 5.0; - separating myrosinase from glucosinolates to provide a myrosinase-containing fraction and a myrosinase-free fraction; and isolating the product from the myrosinase-containing fraction or from the myrosinase-free fraction; wherein the temperature of the disrupted seeds and the aqueous mixture is maintained at a value of from about 0 DEG C to about 65 DEG C. The product may be a protein product or a non-protein product. The processes of the invention provide plant products of use in the animal feeds, non-food products or for human consumption.

Description

field of invention [0001] The present invention relates to a method for the manufacture of a product from a plant material, in particular from seeds of plants from the order Brassicaceae, especially from the family Brassicaceae. The product can be a protein product or a non-protein product. The methods of the invention provide plant products for use in animal feed, non-food products or for human consumption. Non-food products include adhesives, coatings, etc. In particular, the protein product has a reduced content of undesired glucosinolates, phenolic substances and components from non-enzymatic and enzymatic degradation of glucosinolates, and oxidation products of glucosinolates and glucosinolates, Because these components have a negative effect on nutritional value and color. Background of the invention [0002] Methods for producing plant products such as protein isolates or concentrates etc. are well known in the art. Such plant products are typically used in animal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/14A23K1/16A23K1/18A23L1/30A23L1/305
CPCC11B1/04C11B1/10C11B3/16A23K20/147A23K20/10A23L5/273A23L33/30A23L33/105A23L33/15A23L33/185A23L33/22
Inventor 凯尔德·埃德鲁普·安德森克里斯蒂安·巴格尔希尔默·索伦森简斯·克里斯蒂安·索伦森
Owner UNIVERSITY OF COPENHAGEN
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