Application of auranofin to preparation of anti-angiogenic medicine
An anti-angiogenesis and drug technology, applied in the field of medicine, can solve the problems such as anti-angiogenesis of auranofin that have not been seen
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Embodiment 1
[0027] Example 1: Qualitative observation of the inhibitory effect of auranofin on the generation model of zebrafish intersegmental vessels (ISV).
[0028] Zebrafish: The zebrafish used in this example is vascular transgenic green fluorescent zebrafish (a gene specifically expressed by zebrafish endothelial cells acts as a driver to drive the specific expression of green fluorescent protein in zebrafish vascular endothelial cells) ( figure 1 ), the feeding and use standards were strictly in accordance with the requirements of the American Institutional Animal Care and Use Committee (IACUC).
[0029] Fish water preparation method: Add 0.2g of sea salt (Instant Ocean salts) to 1L of deionized water.
[0030] Dimethyl sulfoxide (DMSO, analytical grade): purchased from Sigma-Aldrich (Catalog #101259268, Lot #SHBC2572V).
[0031] Preparation of 0.1% DMSO solution (negative control): When in use, prepare a working solution with a concentration of 0.1% with fish farming water, and p...
Embodiment 2
[0039] Example 2: Quantitative evaluation of the inhibitory effect of auranofin on the generation model of zebrafish intersegmental vessels (ISV).
[0040] Zebrafish vascular endothelial cells sprout from 20hpf after fertilization, form the main intersegmental vascular network at about 30-31hpf, such as dorsal long axis vessels (DLAV) and intersegmental vessels (ISV), and basically form a complete body axis at 48hpf The vascular network, now clearly showing intact intersegmental vessels (ISVs). The complete intersegmental vessel mainly refers to the segment of vessel connecting the dorsal aorta (DA) and the dorsal long axis vessel (DLAV). The 48hpf zebrafish has a total of 28 pairs of intact intersegmental vessels (ISVs).
[0041] The experimental method is as follows:
[0042] (1) Experimental grouping and embryo treatment: 100 well-developed zebrafish embryos were taken, and the embryonic development time was 24 hpf (hour-postfertilization, hpf) after fertilization, and we...
Embodiment 3
[0051] Example 3 Qualitative observation of the inhibitory effect of auranofin on the zebrafish inferior intestinal vessel (SIV) generation model.
[0052] The zebrafish subintestinal vessel (SIV, subintestinal vessel) grows on both sides of the yolk sac, and its shape is like a basket. The length of the subintestinal vessel (SIV) extending downward from the ventral side of the somites is about 50-100 μM. See Figure 4 (72hpf vascular transgenic fluorescent zebrafish subintestinal vascular model).
[0053] The experimental method is as follows:
[0054] (1) Experimental grouping and embryo treatment: 100 well-developed zebrafish embryos were taken, and the embryo development time was 24hpf (hour-postfertilization, hpf) after fertilization, and they were randomly divided into 5 groups (negative control group, three concentrations of drug treatment group, positive control group), and the number of embryos in each group was 20. During the operation, the embryos were evenly dis...
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