Method for improving emission ratio of pinctada maxima parent gamete

Abstract

The invention relates to a method for improving the emission ratio of pinctada maxima parent gametes and belongs to the technical field of shellfish culture. The method concretely comprises the following steps of: selecting individuals with higher activity and plump gonads from a 3-year or 4-year natural population or cultured population as parents; cleaning attachments from the surfaces of shell bodies, and soaking for 1-2 minutes by utilizing 0.02% potassium permanganate solution; soaking the parents in 0.02%-0.05% 5-hydroxytryptamine solutions for 30-50 minutes. The method disclosed by the invention can effectively and fast promote the emission of the pinctada maxima parent gametes and improve the survival rate of the parents and has the advantages of easiness and convenience in operation, low cost, easiness in popularization, and the like.

Description

technical field [0001] The invention relates to a technique for inducing spawning of pearl oysters, in particular to a method for increasing the gamete emission rate of parents of the pearl oyster, and belongs to the technical field of shellfish cultivation. Background technique [0002] Greater Pearl Oyster ( Pinctada maxima Jameson) belongs to Mollusca, Bivalvia, Anisomyaria, Pteriidae, Pinctada, commonly known as Silver lip pearl oyster abroad ) and Golden lip pearl oyster. Living in tropical and subtropical sea areas, it is naturally distributed in the tropical coasts of Australia, the Philippines, Malaysia, Indonesia and other countries. The resources of my country's pearl oyster are mainly distributed in the coasts of Leizhou Peninsula, Hainan Island and Xisha Islands. The pearls cultivated by using the pearl oyster have strong luster, beautiful color, and the diameter is generally more than 1 cm. After processing, each pearl can be worth 3,000-4,000 U.S. dollars, an...

AI Technical Summary

Problems solved by technology

One of the key steps in the breeding of Pinctada maxima is the induction of gametes by the parents. At present, in the seedling breeding process, traditional methods are often used to induce the release of gametes from the parents. The traditional induction mainly includes: 1) Anatomical gonads and artificial insemination. The main defects of this method are: breeding The larvae have poor floating ability, irregular individual development, high deformity rate, and water quality is difficult to control; loss of parental resources; 2) shade drying stimulation; 3) combination of shade drying and temperature difference stimulation

2) and 3) Defects of the method: low induction discharge rate, high mortality after broodstock induction, especially high maturity of broodstock

Invention patent: Application No. 200710168056.8 Provides a land-based controllable water intermediate cultivation method for artificial seedlings with simple operation and low cultivation cost, which solves the problem of low survival rate of Pinctada aquaculture and plays an important role in restoring the cultivation of Pinctada officinalis It has played a positive role. This method solves the problem of the influence of external conditions on the cultivation of Pinctada maxima, and at the same time improves the problem of its survival rate, which is of great significance to the cultivation of Pinctada maxima. , the study on improving the survival rate has not been reported. Therefore, the development of a suitable gamete discharge method for the parents of Pinctada maxima can effectively improve the hatching rate of larvae of Pinctada maxima and increase the seedling quantity per unit of water.