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Protein having antigen activity against liver flukes

A liver fluke and protein technology, applied in the field of protein, can solve problems such as low sensitivity, diagnostic limitations, sensitivity and specificity diagnostic methods cannot be established, and achieve excellent specificity and sensitivity, and great use value

Active Publication Date: 2013-12-18
韩国中央大学校产学协力团
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Antigenicity evaluation research on a small number of proteins has been continuously carried out in the past 10 years, but until now, an excellent single antigen protein with high sensitivity and specificity that can be used as a useful diagnostic reagent has not been developed
In the case of using a single antigen protein, although the specificity is high, the sensitivity is low, so the diagnosis of patients with a low burden of liver fluke infection is limited, etc., making it impossible to establish a diagnostic method with high sensitivity and specificity

Method used

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  • Protein having antigen activity against liver flukes
  • Protein having antigen activity against liver flukes
  • Protein having antigen activity against liver flukes

Examples

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Embodiment approach

[0084] Hereinafter, the present invention will be described in more detail through examples and experimental examples. These Examples and Experimental Examples are only for illustrating the present invention, and therefore, the scope of the present invention is not limited to these Examples and Experimental Examples.

Embodiment 1

[0086] Example 1: cDNA screening of liver fluke antigen candidate protein

Embodiment 1-1

[0087] Example 1-1: Analysis of liver fluke transcriptome information

[0088] 1) Prediction of proteins secreted by liver fluke

[0089] Using the SignalP-natural networks (SignalP-natural networks) and SignalP-hidden Markov model (SignalP-hidden Markov model) program (http: / / www.cbs.dtu. dk / services / SignalP / ) analyzed 12,830 liver fluke transcript cDNA clusters registered in the liver fluke transcriptome library.

[0090] 2) Selection of EST clone (clone)

[0091] Liver fluke transcripts consist of singlets consisting of one read and clusters consisting of more than two reads. When the expressed-sequenced tag (EST, expressed-sequenced tag) predicted to be a secreted protein is a cluster, reads containing a cleavage site were selected (see Figure 1).

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Abstract

The present invention relates to a protein having antigenic activity against liver flukes, and more particularly, to an antigenic protein having enhanced sensitivity and specificity for the diagnosis and treatment of the liver flukes by producing proteins having antigenic activity against the liver flukes. The antigenic protein includes sequences selected from all or parts of amino acid sequences which are selected from the group consisting of SEQ ID Nos. 1 to 4 of the present invention.

Description

technical field [0001] The present invention relates to a protein having antigenic activity useful for the diagnosis and treatment of liver flukes and a gene encoding the antigenic protein. Background technique [0002] When the human body is infected with liver fluke, the liver fluke parasitizes in the bile ducts, causing liver fluke disease. Liver fluke is a chronic parasitic disease prevalent in South Korea. In 2004, the infection rate of liver fluke among Korean nationals was 2.4%. It is estimated that there are about 1.2 million infected people. Moreover, liver fluke is localized in the four major river basins. Infected people continue to emerge. Liver fluke is also endemic in China, Vietnam, Thailand, Cambodia, Laos, Russia and Eastern European countries. [0003] Liver flukes live in the bile ducts of the liver and lay eggs. The eggs will be excreted together with the feces and flow to fresh water, where they are eaten by the snails or several freshwater shellfish ...

Claims

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Application Information

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IPC IPC(8): C07K14/44C12N15/30C07K16/20A61K39/002
CPCA61K39/0003C07K14/43559A61K39/002C07K14/44C07K16/20C12N15/11
Inventor 洪性琮
Owner 韩国中央大学校产学协力团
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