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Application of salidroside in preparation of medicine for treating stroke

A technology for salidroside and stroke, which is applied in the application field of salidroside in the preparation of a drug for the treatment of stroke, and can solve problems such as large individual differences and narrow time windows.

Inactive Publication Date: 2013-12-18
FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the marketed neuroprotective agents for the treatment of stroke have a certain protective effect, there are still large individual differences, and the time window is narrow, usually 4.5-6 hours; even the longest currently available citicoline The effective treatment time window of sodium is only 24 hours, and it takes 3 months to see the effect

Method used

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  • Application of salidroside in preparation of medicine for treating stroke
  • Application of salidroside in preparation of medicine for treating stroke
  • Application of salidroside in preparation of medicine for treating stroke

Examples

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Effect test

Embodiment 1

[0026] Preparation of medicines

[0027] Take 100g of salidroside and 50g of mannitol, dissolve them in 1000ml of water for injection, add water for injection to 1500g, add 8g of activated carbon into the solution, stir and heat to 60°C for 15 minutes, and filter in series with 0.45um and 0.22um filter membranes , to obtain a clarified solution; the clarified solution is divided into control glass bottles for injection according to 1.56g per bottle in a sterile environment, and placed in a freeze-drying box to freeze and dry. The freezing process is to freeze the solution to -40°C and keep it for 3hr. Vacuum down to below 10 Pa, turn on the heating system, sublimate the product temperature below -15°C for 15 hours until the water content of the product sublimates to above 80%, then increase the product temperature to 0°C for secondary drying for 4 hours, and finally the product temperature is at 25°C ℃ drying until the product moisture is lower than 3%; the freeze-dried produc...

Embodiment 2

[0029] Rat middle cerebral artery ischemia (MCAO) reperfusion model, 0, 24 and 48 hours after model establishment, salidroside was injected intravenously for 6 days (50mg / kg / day), and the survival rate and neurological damage score were counted every day. After the experiment, brain tissue was taken for TTC staining and gene chip analysis (microarray).

[0030] For the TTC staining method, please refer to Shen H, Wu X, Zhu Y, Sun H. PLoS One. 2013;8(2):e57055

[0031] For the scoring method of neurological impairment, see the literature Bederson JB, Pitts LH, Tsuji M, Nishimura MC, Davis RL, Bartkowski H. Stroke. 1986;17(3):472-6

[0032] For gene chip analysis methods, see Mengozzi M, Cervellini I, Villa P, Erbayraktar Z, , Yilmaz O, Erbayraktar S, Manohasandra M, Van Hummelen P, Vandenabeele P, Chernajovsky Y, Annenkov A, Ghezzi P. Proc Natl Acad Sci. 2012 12;109(24):9617-22.

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Abstract

The invention relates to the field of medicines, and in particular relates to application of salidroside in preparation of a medicine for treating stroke. The salidroside shows good treatment effect in animal-related model experiments, can effectively increase the score on reduction of nerve function deficit, and also can reduce the volume of cerebral infarction, as well as prolong the stroke therapeutic time window to 48 hours; the salidroside can regulate gene related to nerve synaptic plasticity is proved for the first time.

Description

technical field [0001] The invention relates to medical applications, in particular to the application of salidroside in the preparation of medicines for treating cerebral apoplexy. Background technique [0002] Stroke, commonly known as cerebrovascular accident or cerebral apoplexy, is a sudden onset of cerebral blood circulation disorders and the most serious complication of cerebrovascular diseases. According to statistics, there are 2 million stroke patients in China every year, and the incidence rate is as high as 1.20 / 100,000. Now (2012) there are 7 million surviving stroke patients, 4.5 million of whom have lost their labor force and are unable to take care of themselves to varying degrees. The disability rate is as high as 75%. 1.2 million stroke patients die every year in China. Some scholars have found after analyzing clinical data that about 40% of stroke patients in outpatient clinics are recurrent cases, and 25-33% of stroke patients will have another stroke ...

Claims

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Application Information

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IPC IPC(8): A61K31/7032A61P9/10
Inventor 陈立典洪桂祝吴晓华赖文芳张小琴魏艺聪褚克丹
Owner FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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