Method for detecting distribution of lignin tissue of stone cells of pear fruit

Abstract

The invention relates to a method for detecting distribution of lignin tissue of stone cells of pear fruit. The method comprises the following steps: firstly soaking and treating pear fruit slices with hydrochloric acid, dyeing with 1%-2% KMnO4 solution for detecting distribution of S-lignin tissue, and sealing the slices with ammonia water after dyeing; dyeing with 2% phloroglucinol solution for detecting distribution of G / S-lignin tissue, and sealing the slices with hydrochloric acid after dyeing. With the method, the pear fruit slices are firstly soaked and treated with hydrochloric acid, so that the permeability of the stone cells of the pear fruit slices is improved to be beneficial to coloring lignin monomers with coloring agent. G-lignin and S-lignin in the stone cells of pear are mutually cross-linked and deposited on the cellulose of walls of the stone cells and are not easily distinguished, but the permeability of the stone cells of the pear fruit slices is improved after the pear fruit slices are firstly treated with hydrochloric acid, thus the lignin monomers are more easily colored, and the distribution condition of total lignin and S-lignin monomers can be distinguished well by utilizing different dyeing technologies.

Description

technical field [0001] The invention relates to a lignin detection method, in particular to a method for analyzing the distribution of different lignin monomers in pear fruit stone cells by staining different types of lignin monomers. Background technique [0002] Lignin is generally classified into syringyl lignin (S-lignin), guaiacyl lignin (G-lignin) and p-hydroxyphenyl lignin (H-lignin). G-lignin is generally used in gymnosperms; GS-lignin in angiosperms; GSH-lignin in grasses. Pears are angiosperms. The deposition of lignin on the cell wall of parenchyma cells in pear fruit caused secondary thickening of the cell wall to form stone cells. The distribution of stone cell lignin in pear fruit pulp and the distribution of different lignin monomers all affect the quality of pear fruit. Therefore, there is a need for an accurate, simple and rapid method to detect lignin and the distribution of different lignin monomers in pear fruit pulp. [0003] At present, the analysis...

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Problems solved by technology

[0003] At present, the analysis method for the distribution of stone cell lignin in pear fruit pulp mainly adopts the method of Wu Shaohua et al. to observe slices. This method is difficult to observe the overall distribution of stone cell lignin in pear fruit pulp as a whole, because different types of lignin in pear fruit interact with each other. Together, the different types of lignin monomers in pear fruit stone cells cannot be distinguished

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