Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kit and method for simultaneous detection of Staphylococcus aureus gene and Escherichia coli gene by using dual molecular beacon-LAMP process

A technology of molecular beacons and staphylococci, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of false positives, aerosol pollution, etc., and achieve the effect of simple operation, strong specificity, and high sensitivity

Active Publication Date: 2013-12-11
SHAANXI UNIV OF SCI & TECH
View PDF5 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, LAMP products are susceptible to aerosol contamination, causing false positive results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit and method for simultaneous detection of Staphylococcus aureus gene and Escherichia coli gene by using dual molecular beacon-LAMP process
  • Kit and method for simultaneous detection of Staphylococcus aureus gene and Escherichia coli gene by using dual molecular beacon-LAMP process
  • Kit and method for simultaneous detection of Staphylococcus aureus gene and Escherichia coli gene by using dual molecular beacon-LAMP process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0047] The present invention will be further described in detail below in conjunction with specific drawings and specific embodiments, which are explanations rather than limitations of the present invention.

[0048] The present invention takes Staphylococcus aureus and Escherichia coli O157:H7 as examples to analyze the detection method of the dual molecular beacon-LAMP, so as to realize rapid and accurate simultaneous detection of two pathogenic bacteria.

[0049]According to the conserved sequences of toxin genes or invasion genes of food-borne pathogens published by GenBank, we selected the thermostable nucase Nuc gene of Staphylococcus aureus and the hemolysin gene rbfE of Escherichia coli O157:H7, and designed LAMP primers and Molecular beacon probes, labeled with different fluorescent agents, established a dual molecular beacon-LAMP method to detect the reaction system of these two foodborne pathogens, and applied this detection system to the detection of common bacteria...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a kit and a method for simultaneous detection of a Staphylococcus aureus gene and an Escherichia coli gene by using a dual molecular beacon-LAMP process, belonging to the technical field of molecular biological detection. The kit comprises an LAMP reaction system, wherein the LAMP reaction system comprises a primer group used for detecting the Staphylococcus aureus gene nuc and an rfbE gene of the Escherichia coli O157: H7, the primer group includes a pair of outer primers F3 and B3, a pair of inner primers FIP and BIP and a molecular beacon probe of the nuc gene and further includes a pair of outer primers F3 and B3, a pair of inner primers FIP and BIP and a molecular beacon probe of the rfbE gene. The method provided by the invention has the advantages of high sensitivity, strong specificity, avoidance of cross reaction with a dozen of bacteria of a control group, simple operation, realization of visual and clear observation of results and a fast detection speed.

Description

technical field [0001] The invention belongs to the technical field of molecular biology detection, and relates to a method for simultaneously detecting two kinds of food-borne pathogenic bacteria by a double molecular beacon-loop-mediated isothermal amplification technology, in particular to a double molecular beacon-LAMP method for simultaneous detection of gold A kit and method for the nuc gene of Staphylococcus aureus and the rfbE gene of Escherichia coli O157:H7. Background technique [0002] Food is food to safety first. The problem of food safety has become a major safety hazard that plagues the world, especially food contamination and food-borne diseases caused by pathogenic microorganisms have become a worldwide public health problem. The World Health Organization (WHO) reports that billions of cases of food-borne diseases occur every year in the world. Diarrhea caused by microbial contamination and death. Commonly important pathogenic bacteria in microbial food ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/14C12Q1/10
Inventor 龚频王兰张涛涛陈福欣
Owner SHAANXI UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com