Moesin fragments associated with immune thrombocytopenia

A technique for thrombocytopenia and membrane protein, applied in the fields of molecular biology and medical research, which can solve problems such as delaying appropriate treatment of patients

Active Publication Date: 2013-12-04
SHANGHAI KEXIN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These clinical tests can take months or longer, significantly delaying appropriate treatment for patients

Method used

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  • Moesin fragments associated with immune thrombocytopenia
  • Moesin fragments associated with immune thrombocytopenia
  • Moesin fragments associated with immune thrombocytopenia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0136] Example 1 Generation of moesin fragment series

[0137] The following five moesin fragments were produced:

[0138] a. Moesin 1, comprising amino acids 1-297 of human moesin (SEQ ID NO: 2), close to the N-terminal domain of human moesin;

[0139] b. Moesin-2, comprising amino acids 298-577 of human moesin (SEQ ID NO: 3), close to the helix and C-terminal tail domain of human moesin;

[0140] c. Moesin-3, containing amino acids 298-470 of human moesin (SEQ ID NO: 4), close to the helical domain of human moesin;

[0141] d. Moesin-4, containing amino acids 471-577 of human moesin (SEQ ID NO: 5), proximal to the C-terminal tail domain of human moesin; and

[0142] e. Moesin-5, full length human Moesin, amino acids 1-577 (SEQ ID NO: 1).

[0143] The full-length moesin cDNA sequence (1-1734bp) was obtained from Genebank (Genebank accession number AB527296.1), see image 3 . To generate the desired moesin fragments described above, PCR was used to amplify cDNA fragme...

Embodiment 2

[0146] Example 2 Detection and measurement of specific anti-moesin autoantibodies in the serum of patients with immune thrombocytopenia

[0147] Serum and plasma samples were collected from patients with different stages of immune thrombocytopenia and tested for the presence of anti-moesin autoantibodies that recognize and bind to specific segments of moesin. Patient profiles and clinical information are used to classify patients based on their disease type and stage.

[0148] The moesin fragment obtained in Example 1 was used as the antigen in the ELISA assay against the anti-moesin antibody. Specifically, each microwell of the ELISA plate was coated with about 400ng moesin fragments at 2°C to 8°C for 12-16 hours, then washed once with PBS, then blocked with a blocking solution and stored in vacuum-dried storage for later use. In this way, the highly purified moesin antigen is bound to the wells of a polystyrene microwell plate under the condition of naturally preserving...

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Abstract

The present application provides compositions and methods useful for detecting and monitoring immune thrombocytopenia.

Description

technical field [0001] This application relates to the field of molecular biology and medical research related to autoimmune diseases. More specifically, the present application relates to methods and compositions based on the unique presence of specific autoantibodies associated with immune thrombocytopenia. Background technique [0002] Autoimmune diseases are diseases caused by an abnormal response of the immune system to a patient's own substances and tissues. There are more than 80 different types of autoimmune diseases, which collectively are the second leading cause of chronic disease and the top ten leading causes of death in women in all age groups up to the age of 64 one. [0003] Much effort has been invested in medical research to understand the mechanisms of autoimmune diseases and to find effective diagnostics and treatments. Many autoimmune diseases are currently characterized by the presence and undesirable activity of autoantibodies. These autoantibodies...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/16C07K2/00
Inventor 张玥包骏
Owner SHANGHAI KEXIN BIOTECH
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