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Detection method of organophosphorus pesticide

An organophosphorus pesticide and sample technology, which is applied in biochemical equipment and methods, microbial determination/inspection, and analysis by chemical reaction of materials, etc., can solve the application and promotion of restriction enzyme inhibition biosensors, restriction enzyme inhibition biosensors Problems such as application and measurement results cannot be displayed intuitively, so as to facilitate rapid on-site detection, improve detection selectivity, and be easy to observe.

Active Publication Date: 2013-12-04
CHINA UNIV OF PETROLEUM (EAST CHINA)
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Problems solved by technology

However, this type of detection method also has the following disadvantages: in addition to organophosphorus pesticides, carbamate pesticides also have an inhibitory effect on AChE activity, which determines in principle that this type of sensor lacks selectivity and cannot detect a single or a certain species. Determination of a class of analytes
However, whether it is a single type or an array type enzyme inhibition biosensor, the measurement results cannot be displayed intuitively, and the measured electrical signals, optical signals, or piezoelectric signals need to be converted to complex signals before they can be obtained by the naked eye. Visible measurement signal limits the use of enzyme-inhibited biosensors for rapid detection
Not only that, the detection data obtained by array-type enzyme inhibition biosensors need to be processed by complex pattern recognition calculation methods to finally achieve selective detection, which increases the difficulty of detection and limits the application and promotion of enzyme inhibition biosensors

Method used

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  • Detection method of organophosphorus pesticide

Examples

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Effect test

preparation example Construction

[0037] (3) Preparation of visual sensing array

[0038]After steps (1) and (2) are completed, use a quartz capillary with a diameter of 150-200 μm to spot the dye solution prepared in step (1) onto the polyvinylidene fluoride membrane by manual spotting to form dots. Array (such as 4×4, 5×5, 6×6 lattice), put it in a desiccator under the protection of nitrogen until it is completely dry, take out the membrane and put it into the sodium hydroxide prepared in step (2) for treatment solution, soak for 5-10 minutes, carry out alkaline treatment, take out the diaphragm, put it in a desiccator under nitrogen protection until it is completely dry, and prepare a visual sensing array.

[0039] (4) Preparation of AChE enzyme solution

[0040] Add distilled water to AChE (500UN), shake and dissolve, and then prepare AChE enzyme solution with an activity unit of 55-60UN.

[0041] (5) Preparation of thioacetylcholine chloride solution

[0042] Distilled water is added into the thioacety...

Embodiment 1

[0063] (1) Preparation of dye solution

[0064] With 14 kinds of pH indicators (including bromocresol green, bromocresol purple, Congo red, chlorophenol red, bromophenol blue, bromophenol red, metamine yellow, methyl red, bromothymol blue, cresol red , bright yellow, nitrazine yellow, thymol blue and indigo carmine) and 2 kinds of porphyrin derivatives (including tetraphenylporphyrin monosulfonate and tetraphenylporphyrin tetrasulfonate) and other 16 kinds of dye substances As the sensing material, with absolute ethanol as solvent, according to the quality of the dye: the volume ratio of absolute ethanol is the ratio of 1:0.6, after adding absolute ethanol to the dye, stirring and fully dissolving, 16 kinds of mass concentrations are prepared respectively. 1.7g / L dye solution, stored at 3°C ​​in the dark.

[0065] (2) Preparation of sodium hydroxide treatment solution

[0066] Add distilled water to the sodium hydroxide, stir and fully dissolve, and prepare a sodium hydroxid...

Embodiment 2

[0092] A visual rapid identification method for micro-trace organophosphorus pesticides, the specific method steps are the same as those in Example 1, wherein:

[0093] In step (1): according to the quality of the dye: the volume ratio of absolute ethanol is 1:0.3, add absolute ethanol to the dye, stir and fully dissolve, and prepare dye solutions with a mass concentration of 5.1g / L respectively , stored at 3.5°C in the dark.

[0094] In step (2): add distilled water to the sodium hydroxide, stir and dissolve fully, and prepare a sodium hydroxide treatment solution with a concentration of 0.001 mol / L.

[0095] Step (3): use a quartz capillary with a diameter of 180 μm to spot the dye solution prepared in step (1) on the polyvinylidene fluoride membrane by manual spotting to form a 4×4 lattice, and put After completely drying in a desiccator under the protection of nitrogen, take out the membrane and put it into the sodium hydroxide treatment solution prepared in step (2), soa...

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Abstract

The inventive aims to overcome the defects of the conventional enzyme inhibition-type biosensor and provides a visual identification method for detecting a micro-trace organophosphorus pesticide. When the detection method is adopted to detect the organophosphorus pesticide, on one hand, an adopted AChE liquid (Acetylcholine Esterase) is not subjected to solidification treatment, so that the highest activity of the AChE liquid is kept, and the detection sensitivity is improved; on the other hand, the extraction and recovery rate of the organophosphorus pesticide in a sample to be detected is improved through an adopted extracting solution, so that the detection sensitivity is improved to 0.01 ng / L. The detection method can be extensively applied to the detection of micro-trace organophosphorus pesticide residue in grains, vegetables, fruits and other agricultural products as well as the environment, and can also be used in the safety evaluation of agricultural products and the environment, the pollution condition monitoring of the organophosphorus pesticide, and the like.

Description

technical field [0001] The invention belongs to the technical field of organophosphorus substance detection, and in particular relates to a method for detecting organophosphorus pesticides, that is, a method for visually and rapidly detecting organophosphorus substances. Background technique [0002] Organophosphorus pesticides have become one of the three pillars of pesticides in the world because of their many control objects, wide application range, and low price, and they play a huge role in stable and high agricultural production. However, since most organophosphorus pesticides are highly toxic compounds, they not only pollute natural resources such as soil and water sources, but also accumulate in animals and plants, and pass through the food chain, becoming a hidden danger that endangers people's lives. The food safety problem caused by organophosphorus pesticide residues is one of the most important threats to public health at present, and poisoning incidents occur f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/46G01N21/80
Inventor 杨丽敏姜磊刘振李友训
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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