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Glass chip for cultivating single cell array based on microfluidic patterning technology and preparation method thereof

A glass chip, single-cell technology, applied in tissue cell/virus culture devices, biochemical equipment and methods, biochemical instruments, etc., can solve the problem that the position and shape of cell growth cannot be restricted, single-cell arrays cannot be obtained, and closed cells cannot be formed. Area and other issues, to achieve the effect of easy control of parameters, simple and easy operation of the preparation process, and high economy

Inactive Publication Date: 2013-12-04
NORTHWESTERN POLYTECHNICAL UNIV
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  • Application Information

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Problems solved by technology

[0005] The following problems exist in the prior art: the cell growth substrate is chemically modified to promote cell adhesion based on microfluidic patterning technology, and what is obtained is a strip-shaped modified pattern that is the same as the planar structure of the microchannel network, and cannot form a closed area. Therefore, The surface modification obtained by this method cannot restrict the growth location and shape of cells, and cannot obtain single-cell arrays

Method used

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  • Glass chip for cultivating single cell array based on microfluidic patterning technology and preparation method thereof
  • Glass chip for cultivating single cell array based on microfluidic patterning technology and preparation method thereof
  • Glass chip for cultivating single cell array based on microfluidic patterning technology and preparation method thereof

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Embodiment 1

[0026] The chip in this example is used to culture a single osteoblast MC3T3-E1 for the study of cell contact connection. MC3T3-E1 average diameter is 20 μm.

[0027] refer to Figure 1 ~ Figure 3 . In this example, the single cell array culture glass chip was used for MC3T3-E1 cell culture. Among them, the structural dimensions of the single-cell array culture glass chip are as follows: the size of the cell growth region 1 a=b=50 μm; the lateral distance w1 between the centerlines of two adjacent cell growth regions 1=70 μm; The longitudinal spacing w2 between the center lines of area 1 = 80 μm; the line width d of PHEMA hydrogel pattern 2 = 20 μm, and the size of the raised part of the hydrogel pattern c = e = 20 μm; the maximum distance between two adjacent PHEMA hydrogels The narrow distance h=20 μm.

[0028] In this embodiment, based on microfluidic patterning technology, the preparation method of single cell array culture glass chip is realized, which is completed ...

Embodiment 2

[0037] The chip in this example is used to culture human mammary epithelial cells HMEC to study the growth characteristics of the cells. HMEC mean diameter is 45 μm.

[0038] refer to Figure 1 ~ Figure 3 . In this example, the single cell array culture glass chip was used for HMEC cell culture. Among them, the structural dimensions of the single-cell array culture glass chip are as follows: the size of the cell growth region 1 a=b=80 μm; the lateral distance w1 between the centerlines of two adjacent cell growth regions 1=120 μm; The longitudinal spacing w2 between the centerlines of area 1 = 140 μm; the line width d = 40 μm of the PHEMA hydrogel pattern, the size of the raised part of the hydrogel pattern c = e = 40 μm; the maximum distance between two adjacent PHEMA hydrogels The narrow distance h=20 μm.

[0039] In this embodiment, based on microfluidic patterning technology, the preparation method of single cell array culture glass chip is realized, which is complet...

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Abstract

The invention discloses a glass chip for cultivating a single cell array based on microfluidic patterning technology, and a preparation method thereof, and belongs to the field of biological micro electro mechanical systems. The glass chip adopts the structure that a layer of PHEMA hydrogel pattern 2 is arranged on a glass base 4, and divides the glass base 4 into a plurality of rectangular cell growth zones 1 to generate the single cell array. The preparation process is that graphical PHEMA processing is performed on the surface of a glass sheet through a PDMS elastic stamp. The glass chip has the benefits that the stable surface chemical modification is adopted, so that hydrogel dressings capable of resisting cell adhesion is arranged on the surface of the glass in a graphical manner to form the cell graphical surface containing cell adhesion and resisting cell adhesion so as to cultivate the single cell array; the preparation process is simple and easy to operate; when the chip is applied to cell culture, the single cell growth array is obtained, and a new tool for studying basic cell biology is provided.

Description

technical field [0001] The invention relates to a single-cell array culture glass chip structure and preparation method based on microfluidic patterning technology (microfluidic patterning, uFLP), and belongs to the field of biological micro-electromechanical systems (Bio-MEMS). Background technique [0002] Cell culture is one of the most important basic sciences in the fields of biology, medicine, and new drug development. Over the past two hundred years, cell culture has made great contributions to vaccine development, genetic analysis, and biological research. With the development of modern biochemistry, molecular biology, molecular genetics, and modern medicine, cell culture has also fully demonstrated its great development potential in many application fields. There are many conventional culture methods for adherent cells, which are characterized by cell population culture, such as cell culture flasks, cell culture dishes, and multiwell cell culture plates. However, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00
Inventor 高洁叶芳马炳和魏晨谢丽郝艳鹏谢晋
Owner NORTHWESTERN POLYTECHNICAL UNIV
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