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Method for preparing tumor-specific DC vaccine by applying CD34+ cells of umbilical cord blood

A tumor-specific, cell-based technology, which is applied in the field of preparation of tumor-specific DC vaccines using umbilical cord blood CD34+ cells, can solve the problems of restricting clinical applications, mass production of DC vaccines, and industrial production of DC vaccine preparations.

Active Publication Date: 2014-12-10
玥特农生物科技河北有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the individual characteristics of PBMC-derived DCs (PBMC-DCs), clinical applications are limited, especially the mass production of DC vaccines and the industrial production of DC vaccine preparations.

Method used

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  • Method for preparing tumor-specific DC vaccine by applying CD34+ cells of umbilical cord blood
  • Method for preparing tumor-specific DC vaccine by applying CD34+ cells of umbilical cord blood
  • Method for preparing tumor-specific DC vaccine by applying CD34+ cells of umbilical cord blood

Examples

Experimental program
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Embodiment 1

[0069] ①. Fresh tumor tissue resected by surgery: take 0.3-1.0 cm of tissue around the tumor (to avoid necrotic tissue in the center of the tumor) 3 (It can be stored in liquid nitrogen or -80°C refrigerator for later use), cut off the surrounding non-tumor tissue, washed 3-5 times with gentamicin-normal saline, cut into pieces, grinded with a 300-mesh steel mesh to prepare a single-cell suspension, and freeze-thawed repeatedly The tumor cell lysate was prepared by the method, and the protein was quantified after passing through the mesh for later use.

[0070] ②Tumor cells obtained from pleural and ascites: Take 1500-2000ml of pleural and ascites, centrifuge at 1200rpm, wash and centrifuge with normal saline for 3 times, pass through a 300-mesh steel mesh to obtain a single cell suspension, and prepare tumor cell lysate by repeated freezing and thawing , protein quantification after passing through the screen for later use.

[0071] ③. Cell lines derived from the same tissue...

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Abstract

The invention discloses a method for preparing a tumor-specific DC vaccine by applying CD34+ cells in umbilical cord blood. The method comprises (1) a step of preparing autologous tumor-related holoantigen; (2) a step of obtaining the umbilical cord blood; (3) a step of obtaining mononuclear cells derived from the umbilical cord blood; (4) a step of purifying CD34+ cells in the mononuclear cells derived from the umbilical cord blood; (5) performing induction culture for a precursor DC; (6) a step of performing amplification and culture of an immature DC; and (7) a step of preparing the DC vaccine.

Description

technical field [0001] The invention relates to a preparation method of a vaccine, in particular to a method for preparing a tumor-specific DC vaccine using umbilical cord blood CD34+ cells. Background technique [0002] Cellular immunotherapy of tumors mainly relies on DC-induced T cell immunity. DC is a professional antigen-presenting cell in the human body. Therefore, in tumor immunotherapy, it undertakes the important function of ingesting and processing tumor antigens, and presenting antigen information to T cells, thereby initiating tumor-specific T cell immunity. [0003] DC cells currently used in clinical practice are mainly derived from peripheral blood mononuclear cells (PBMC), and are mainly used in individualized cellular immunotherapy. Due to the individual characteristics of PBMC-derived DC (PBMC-DC), clinical applications are limited, especially the mass production of DC vaccines and the industrial production of DC vaccine preparations. [0004] The patent ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/00C12N5/0784A61P35/00
Inventor 蔡建辉刘刚陈宝平袁灿冯瑞城
Owner 玥特农生物科技河北有限责任公司
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