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Pharmaceutical composition for promoting wound healing, and appliacations of sambucus plant or isatis plant for preparing medication for promoting wound healing

A medical composition and wound healing technology, which is applied in the direction of medical preparations containing active ingredients, drug combinations, and pharmaceutical formulas, and can solve problems such as the inability to infer the relationship between Banlan

Inactive Publication Date: 2013-10-30
IND TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to some published literature, it is clear that many medicines or Chinese herbal medicines have the effects of "anti-inflammation" and "inhibition of angiogenesis" (http: / / tcam.ccmp.gov.tw / infomation / 95 / CCMP94-RD- 016.pdf; http: / / ir.ym.edu.tw / ir / handle / 987654321 / 4458 ; http: / / ir.lib.csmu.edu.tw:8080 / ir / handle / 310902500 / 726), however, the content disclosed in the previous technology has no way to infer the relationship between Banlana and bone disappearance and the promotion of angiogenesis

Method used

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  • Pharmaceutical composition for promoting wound healing, and appliacations of sambucus plant or isatis plant for preparing medication for promoting wound healing
  • Pharmaceutical composition for promoting wound healing, and appliacations of sambucus plant or isatis plant for preparing medication for promoting wound healing
  • Pharmaceutical composition for promoting wound healing, and appliacations of sambucus plant or isatis plant for preparing medication for promoting wound healing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Embodiment 1: preparation of plant extract

[0086] 1. Ethanol extraction of medicinal materials

[0087] Add 200g of the whole plant powder of Guxiao, 200g of the root of Guxiao, 200g of the stem of Guxiao and 200g of the root of Isatis indigo (Northern Banlan) into 5 times the volume of 95 % ethanol, and extracted by shaking at 120rpm. After 7 days, the obtained extract was air-filtered. Then, the filtrate was concentrated under reduced pressure to about 30 mL, and distributed into freeze-drying bottles for freeze-drying, and the obtained extract was weighed and recorded. Freeze-dried extracts were stored at 4°C or -20°C.

Embodiment 2

[0088] Example 2: Identification of Plant Origins

[0089] 1. DNA extraction

[0090] DNA extraction was carried out on Guxiao and Isatis indigo respectively to obtain their individual DNA. The DNA extraction steps are as follows:

[0091] (1) Put an appropriate amount of plant samples in a mortar, add liquid nitrogen and grind them into powder.

[0092] (2) Pour an appropriate amount of powder sample into a centrifuge tube containing 5-10ml of DNA extraction buffer (sample volume and buffer base can be adjusted according to demand), mix well, and place in a 65°C water bath for 30-60 minutes. Take out and shake evenly 2-3 times.

[0093] (3) After taking it out, add a solution of chloroform / isoamyl alcohol (24:1 (v / v)) equal to the volume of the extraction buffer, mix well, and set the temperature at 8,000rpm at 4°C Centrifuge for 5 minutes.

[0094] (4) The supernatant was taken out and divided into new microcentrifuge tubes, 0.6 times the volume of isopropanol (isopropa...

Embodiment 3

[0122] Example 3: Plant extracts promote human umbilical vein endothelial cells (Human Umbilical Vein Endothelial Cells, HUVEC) cell proliferation-MTT analysis

[0123]

[0124] Screening of extracts that promote the growth of umbilical vein endothelial cells. Using human vascular endothelial cells, the growth factor VEGF or Notoginsenoside R1 (Notoginsenoside R1) and other Chinese herbal medicine extracts are used as the control group to screen for drugs that promote the growth of vascular endothelial cells with activity evaluation platform. The implementation method is to put about 5x10 3 Cells / well of HUVEC were seeded in gelatin-coated 96-well plates with M199 complete medium in 5% CO 2 overnight culture in a 37°C incubator; the next day, replace it with a Chinese herbal medicine extract containing the quantitative concentration to be tested and a fresh 5% serum medium without ECGS, and then place the 96-well plate in the incubat...

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Abstract

The invention provides a pharmaceutical composition for promoting wound healing, including an effective amount of a Sambucus plant or an Isatis plant as an active ingredient for promoting wound healing; and a pharmaceutically acceptable carrier or medium.

Description

technical field [0001] The present invention relates to a pharmaceutical composition with the effect of promoting wound healing, and in particular to a pharmaceutical composition for promoting wound healing, which uses Sambucus formosana as the main active ingredient, and may further include Beiban Blue (Isatis indigotica) as one of the active ingredients. Background technique [0002] With the development of the global aging society, the incidence of wound infection and complications increases, which makes the wound pattern increasingly complex, and often develops into chronic wound, which has become a difficulty in current medical care and treatment. Causes of chronic wounds include, for example, Diabetic Ulcers / Neuropathic Ulcers, Pressure / Decubitus Ulcers, Arterial / Venous Ulcers, etc. [0003] Taking diabetic patients with chronic wounds as an example, 25% of these patients will have foot ulcers, difficult wound healing, and easy infection. Once the above symptoms occur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/35A61K36/315A61P17/02
CPCA61K36/185A61K36/315A61P17/02
Inventor 蔡佩宜曾湘文陈燕春徐惠纯温奕泓王俊中邱淑娇
Owner IND TECH RES INST
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