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Chorispora bungeana ferroportin gene CbIRT and amino acid sequence of encoding product of Chorispora bungeana ferroportin gene CbIRT

A technology of iron transporter and alpine ion mustard, applied in genetic engineering, plant gene improvement, plant peptides, etc., can solve problems such as drought stress and poor water retention of gravel

Inactive Publication Date: 2013-10-23
LANZHOU UNIVERSITY
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  • Abstract
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  • Application Information

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Problems solved by technology

And on the windward slope, the gravel in the flow stone has poor water retention, which often causes drought stress

Method used

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  • Chorispora bungeana ferroportin gene CbIRT and amino acid sequence of encoding product of Chorispora bungeana ferroportin gene CbIRT
  • Chorispora bungeana ferroportin gene CbIRT and amino acid sequence of encoding product of Chorispora bungeana ferroportin gene CbIRT
  • Chorispora bungeana ferroportin gene CbIRT and amino acid sequence of encoding product of Chorispora bungeana ferroportin gene CbIRT

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Experimental program
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Embodiment 1

[0015] Alpine ion iron transporter gene ikB sequence cloning method

[0016] High-quality total RNA of Jasmine japonica, which meets the requirements of reverse transcription template, was obtained by Trizol method. According to the sequence of plant iron transporter gene published in GeneBank, degenerate primers were designed to amplify the conserved region of CDS (B=T+G+C; Y =T +C; D =A +T+G; N = A+C+G +T; R =A +G) to get the target fragment of 487 bp. According to the sequencing results and sequence alignment results of the first cloned fragment, P3 and P4 primers were designed using the iron transporter gene of Arabidopsis thaliana as a template. Using P3 and FED131 as primers to amplify, an obvious target band of 440 bp was obtained. The other end was amplified with FEU204 and P4 primers to obtain a 544 bp target band. The sequencing results of the three PCR products were spliced ​​to obtain the complete CDS sequence. The ferroportin gene ikB Has the nucleotide sequ...

Embodiment 2

[0029] alpine ion mustard transporter gene ikB Preliminary identification of functions

[0030] Alpine mustard was treated with different iron ion concentrations (100 μM, 200 μM and 500 μM). Determination of 12 h, 24 h, 36 h in different treatments of Alpine ion mustard ikB gene expression. The measurement result shows ( image 3 ), under Fe concentration of 100 μM and 200 μM treatment, ikB The expression showed the same changing trend, and the expression level gradually increased with time. At 36 h, the first two Fe concentrations ikB The expression levels of each reached 2.2 and 1.8 times of the control level, respectively. At 500 μM Fe concentration ikBThe expression level of the protein showed a decreasing trend, and the expression level was lower than the control level within 36 h. The experimental results show that in a certain concentration of iron ion environment, ikB induced expression occurs, indicating that ikB It is related to the iron tolerance of Alpine...

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Abstract

The invention provides a Chorispora bungeana ferroportin gene CbIRT which has a base sequence shown as SEQ ID NO:1. The invention also provides an encoding product CbIRT of the Chorispora bungeana ferroportin gene CbIRT, wherein the encoding product CbIRT has an amino acid sequence shown as SEQ ID NO:2.

Description

technical field [0001] The invention relates to the field of plant genetic engineering, in particular to a high mountain ion mustard iron transporter ikB The amino acid sequence of a gene and its encoded product. Background technique [0002] Mineral nutrition has an extremely important impact on plant growth and development. Of all the essential elements in plants, iron's redox properties endow it with an important role in a variety of enzymes. Although iron is abundant in the earth's crust, almost all iron in soil is in the oxidized form Fe(III), thus plants need to employ efficient mechanisms to obtain iron from soil. Iron transporter (IRT) is a member of the metal ion transporter family - ZIP family. These proteins have similar transmembrane structures, conserved sequences and recognition sequences. In Arabidopsis, the expression of IRT1 is regulated at both transcriptional and protein levels. [0003] Alpine ion mustard (Chorispora bungeana) is a perennial plant of ...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415
Inventor 安黎哲韩艳李争艳徐世健向云张华盛红梅陈书燕何文亮李田
Owner LANZHOU UNIVERSITY
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