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Method for extracting blueberry anthocyanin

A blueberry anthocyanin and extraction method technology, which is applied in the field of blueberry anthocyanin extraction, can solve the problems of loss in the extraction process and low concentration of blueberry anthocyanin extract, and achieve environmental protection, overcome the large amount of solvent used, The effect of significant economic and social benefits

Inactive Publication Date: 2013-10-09
青岛蓝莓生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the deficiencies in the prior art, the technical problem to be solved by the present invention is to provide a method for extracting blueberry anthocyanins, which solves the problems of low concentration of blueberry anthocyanin extracts and large losses during the extraction process.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The extraction method of blueberry anthocyanin of the present embodiment, it comprises the following steps:

[0015] (1) Pretreatment of raw materials: take non-rotten blueberry fruit, wash and remove impurities, add extract solution according to the mass ratio of material to liquid at 1:1, crush and beat to obtain blueberry slurry; the extract solution is ethanol solution acidified by citric acid; The volume fraction of ethanol in the extract is 35%, and citric acid is added dropwise to make the acidified ethanol solution have a pH value of 3.0-4.0.

[0016] (2) Extraction of blueberry anthocyanins: take the blueberry slurry, add the aforementioned extract according to the volume ratio of material to liquid 1:4, stir and extract for 20-30 minutes, then let it stand for precipitation and layering, then extract at 40-65°C for 3 hours , collecting the upper layer extract; the extraction is ultrasonic-assisted extraction; ultrasonic temperature is 30-50°C, ultrasonic power...

Embodiment 2

[0019] The extraction method of blueberry anthocyanin of the present embodiment, it comprises the following steps:

[0020] (1) Pretreatment of raw materials: take the blueberry fruit without rot, wash and remove impurities, add the extract solution according to the mass ratio of material to liquid at 1:2, break and beat to obtain blueberry slurry; the extract solution is an ethanol solution acidified by tartaric acid; extract The volume fraction of ethanol in the solution is 75%, and tartaric acid is added dropwise to make the pH of the acidified ethanol solution 5.0-6.0.

[0021] (2) Extraction of blueberry anthocyanins: take blueberry slurry, add the aforementioned extract according to the volume ratio of material to liquid 1:10, stir and extract for 20-30 minutes, then let it stand for precipitation and layering, then extract at 40-65°C for 2 hours , collecting the upper layer extract; the extraction is ultrasonic-assisted extraction; ultrasonic temperature is 30-50°C, ult...

Embodiment 3

[0024] The extraction method of blueberry anthocyanin of the present embodiment, it comprises the following steps:

[0025] (1) Pretreatment of raw materials: take the non-rotten blueberry fruit, wash and remove impurities, add the extract according to the mass ratio of material to liquid at 1:1.5, break and beat to obtain blueberry slurry; the extract is an ethanol solution acidified by lactic acid; extract The volume fraction of ethanol in the solution was 50%, and lactic acid was added dropwise to make the pH value of the acidified ethanol solution 5.0.

[0026] (2) Extraction of blueberry anthocyanins: take the blueberry slurry, add the aforementioned extract according to the volume ratio of material to liquid 1:7, stir and extract for 20-30 minutes, then let it stand for precipitation and layering, then extract at 40-65°C for 2.5 hours , collecting the upper layer extract; the extraction is ultrasonic-assisted extraction; ultrasonic temperature is 30-50°C, ultrasonic powe...

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Abstract

The invention discloses a method for extracting blueberry anthocyanin. The method comprising the following steps of: (1) pretreating raw materials; (2) extracting blueberry anthocyanin to obtain a blueberry anthocyanin crude extraction solution; and (3) refining the blueberry anthocyanin, and performing vacuum concentration on the blueberry anthocyanin until the content of the blueberry anthocyanin is 50% to obtain a finished product of a blueberry anthocyanin concentrated solution. According to the method for extracting the blueberry anthocyanin, the problems of low concentration of a blueberry anthocyanin extract and higher loss of the blueberry anthocyanin during extraction can be solved, and the defects of large solvent usage amount, long extraction time and high production cost of the existing extraction method can be overcome, so that the method disclosed by the invention is beneficial for environmental protection, energy conservation and development of cyclic economy, and has very significant economic and social benefits.

Description

technical field [0001] The invention relates to an extraction method of blueberry anthocyanins, in particular to an extraction method of blueberry anthocyanins with an anthocyanin content of 50%, and belongs to the technical field of extraction of natural active substances. Background technique [0002] Blueberry is a shrub, with 25-30 fruiting branches per cluster. It blooms and bears fruit in the second year, but the yield is small. It has a proper yield in the third year, and enters the high yield period in the fifth year. -6 kg, the average fruit weight is 2 grams, generally about 300 plants are planted per mu, and the average yield is 1500-2000 kg. Anthocyanin is a pure natural anti-aging nutritional supplement. Studies have shown that it is the most effective antioxidant found by humans today. Its antioxidant performance is 50 times higher than vitamin E and 200 times higher than vitamin C. It is 100% bioavailable to the human body and can be detected in the blood twe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62
Inventor 张春来
Owner 青岛蓝莓生物科技有限公司
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