Preparation method of high-purity carnosol

Abstract

The invention relates to a preparation method of a natural active compound. The preparation method provided by the invention comprises the following steps: 1, extracting a dry carnosol-containing raw material per kilogram by the use of 3-10 liters of a lipophilic organic solvent for 2-5 times, merging extracts, and condensing to obtain a carnosol-containing extract or solid; 2, dissolving by the use of a 10-90% hydrophilic organic solvent, filtering and precipitating, and carrying out macroreticular resin refining processing on a filtrate to obtain a carnosol-containing precipitate; 3, dissolving the precipitate by the use of a mixed lipophilic organic solvent, standing, precipitating a precipitate; and filtering the precipitate to obtain a carnosol crude product; and 4, crystallizing by the use of a hydrophilic organic solvent so as to obtain high-purity carnosol crystals with the HPLC detection content being more than 95%. The preparation method provided by the invention has advantages of simple operation process and controllable cost, and is suitable for large-scale preparation and production of high-purity carnosol.

Description

technical field [0001] The invention relates to a preparation method of a natural active compound. Background technique [0002] rosemary( Rosmarinus officinalis Carnosol and other phenolic diterpenoids contained in plants such as L. are recognized as natural antioxidants in recent years and are widely used in the fields of food industry, daily chemical industry, medicine and health care; and studies have shown that carnosol Turmeric phenol can have anti-tumor effect by inhibiting NF-kB channel, so it is a natural active compound favored by new drug researchers in recent years. [0003] Regarding the preparation of carnosol, in the Chinese patent CN101040901A, a rosemary extract containing 5-12% carnosol is obtained by solvent extraction, cold precipitation, and activated carbon decolorization of rosemary; China In the patent CN101402864A, through the process of extracting the essential oil of rosemary with water vapor first, then extracting the residue with organic solve...

AI Technical Summary

Problems solved by technology

In the Chinese patent CN102115442A, by extracting rosemary with a solvent and performing phosphoric acid-silica gel column chromatography under nitrogen protection, carnosol with a content of 92% is obtained. Although this method can obtain sage with a higher purity carnosol, but the conditions used are too harsh, the silica gel needs to be phosphorylated to make phosphoric acid-silica gel, and the chromatography process needs nitrogen protection, so it is difficult to prepare a large amount of carnosol, and it is also difficult to popularize and practically apply this special method

However, high-purity carnosic acid is required as a raw material. Carnosic acid is also an active compound extracted and isolated from plants such as rosemary. If high-purity carnosic acid is used for peroxidation to obtain high-purity carnosol, Then its source will be greatly limited, and manufacturing cost is very high, if make carnosol with the carnosic acid that purity is not high, then the yield of the carnosol that prepares is low, be difficult to obtain a large amount of target substances, therefore, The method of obtaining high-purity carnosol by peroxidation of carnosic acid has great difficulties in terms of scale and cost control, and is difficult to be practically applied