Viscosity-reducing equipment and method for viscous clinical test specimens

Abstract

The invention relates to viscosity reducing equipment and method for viscous clinical examination specimens and belongs to the technical field of clinical examination. The viscosity reducing equipment for viscous clinical examination specimens comprises a reagent bottle, a viscosity reducing reagent and a plurality of glass beads, wherein the viscosity reducing reagent is put in the reagent bottle and comprises a neutral phosphate buffer solution, protease and normal saline, and the glass beads are put in the reagent bottle. The viscosity reducing method comprises the following steps of: putting the viscous clinical examination specimens into the viscosity reducing equipment and shaking for 1 to 2 minutes to obtain low-viscosity clinical examination specimens. According to the viscosity reducing equipment and the viscosity reducing method, the viscous clinical specimens are changed into diluted specimens by utilizing the shearing force generated among the glass beads during violent movement and the enzyme degradation of the protease, so that pathogenic bacteria in the specimens are dissociated completely and the culture positive rate of the pathogenic bacteria is improved, and the viscosity of the viscous specimens can be reduced only within 1 to 2 minutes, and the viscosity reducing time is obviously shortened.

Description

technical field [0001] The invention belongs to the field of clinical testing, in particular to a method for reducing viscosity of viscous clinical testing specimens. Background technique [0002] Clinical examination is to test the patient's blood, body fluids, secretions, excretions and exfoliations through visual observation, physical, chemical, instrumental or molecular biological methods. Clinical examination provides an important basis for clinical diagnosis. [0003] Clinical test samples such as sputum, pus, semen, viscous vaginal secretions, pus or pus and blood are relatively viscous, and pretreatment is usually required before testing. At present, the method for pretreatment of the above-mentioned clinical test specimens for bacterial culture is as follows: add the viscous specimens into a test tube containing 15-20ml sterilized normal saline, vibrate vigorously for 5-10 seconds, take out the viscous specimens with an inoculation loop and use the same The method...

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Problems solved by technology

[0004] The existing pretreatment methods for viscous clinical test specimens have the following problems: the specimens used for bacterial culture are still viscous after treatment, and the pathogenic bacteria cannot be fully dissociated, which affects the inoculation effect, and eventually leads to the loss of the specimens used for bacterial culture. The positive rate is low; the sample used for PCR detection can be well reduced by NaOH treatment, but it takes a long time, and after being treated with strong alkaline solution, it cannot be directly used for PCR detection, and must be washed with neutral solution 2-3 times to neutralize, repeated washing will lead to the loss of pathogenic bacteria, and eventually lead to a decrease in the number of PCR amplification copies and a decrease in the positive rate; the above two methods are more complicated to operate and require professionals to operate, so they cannot be used at any time Fast and efficient processing of specimens anywhere

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