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Production method for extracting and purifying hyaluronic acid

A technology of hyaluronic acid and production method, applied in the field of microorganisms and biological products, can solve the problems of low product yield, long process lines, many human factors, etc., and achieve the advantages of shortening process lines, reducing production costs and saving costs and expenses. Effect

Inactive Publication Date: 2013-09-18
SICHUAN HENGYI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The object of the invention of the present invention is: for existing CPC complexing ethanol precipitation technology purification, there are long process line, long production cycle, high labor intensity, many human factors affecting product quality, and relatively low product yield, Provide a production method for extracting and purifying hyaluronic acid

Method used

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  • Production method for extracting and purifying hyaluronic acid
  • Production method for extracting and purifying hyaluronic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Fermentation broth inactivation: put the fermentation broth into the first fermentation tank, stir, and the stirring speed is 60 rpm, then add trichloroacetic acid to the first fermentation tank, adjust the pH value of the fermentation broth to 4.0, stir for 60min, The enzyme in the fermentation broth is inactivated, and the acidic protein in it reaches the isoelectric point to aggregate and precipitate, and the inactivated fermentation broth is obtained. In this step, using trichloroacetic acid to adjust the pH value of the fermentation broth can inactivate the fermentation broth and precipitate acidic proteins.

[0038] Primary alcohol precipitation: transfer the inactivated fermentation broth to the first settling tank, slowly add 95% ethanol twice the volume of the inactivated fermentation broth to the first settling tank, and turn on the air flotation device in the first settling tank , while stirring for 30-60min.

[0039] Coarse separation: after the stirring is...

Embodiment 2

[0051] Fermentation broth inactivation: put the fermentation broth into the first fermenter, stir, and the stirring speed is 40 rpm, then add trichloroacetic acid to the first fermenter, adjust the pH value of the fermented broth to 4.5, stir for 80min, The enzyme in the fermentation broth is inactivated, and the acidic protein in it reaches the isoelectric point to aggregate and precipitate, and the inactivated fermentation broth is obtained.

[0052] Primary alcohol precipitation: transfer the inactivated fermentation broth to the first settling tank, slowly add 2.2 times the volume of inactivated fermentation broth with 95% ethanol to the first settling tank, and turn on the air flotation device in the first settling tank , while stirring for 50min.

[0053] Coarse separation: after the stirring is completed, collect the crude hyaluronic acid product floating in the inactivated fermentation broth and the crude hyaluronic acid product at the bottom of the first settling tank...

Embodiment 3

[0062] Fermentation broth inactivation: put the fermentation broth into the first fermentation tank, stir, and the stirring speed is 100 rpm, then add trichloroacetic acid to the first fermentation tank, adjust the pH value of the fermentation broth to 4.0, stir for 30min, The enzyme in the fermentation broth is inactivated, and the acidic protein in it reaches the isoelectric point to aggregate and precipitate, and the inactivated fermentation broth is obtained.

[0063] Primary alcohol precipitation: transfer the inactivated fermentation broth to the first settling tank, slowly add 2.5 times the volume of inactivated fermentation broth with 95% ethanol to the first settling tank, and turn on the air flotation device in the first settling tank , while stirring for 30min.

[0064] Coarse separation: after the stirring is completed, collect the crude hyaluronic acid product floating in the inactivated fermentation broth and the crude hyaluronic acid product at the bottom of the...

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Abstract

The invention discloses a production method for extracting and purifying hyaluronic acid, for solving the problems such as long process route, long production period, high labor intensity, more human factors influencing product quality and relatively low product yield, existing in the purification of the traditional CPC (Cetyl Pyridinium Chloride) complexing ethanol sedimentation technology. The production method comprises the following steps of: fermentation liquor inactivation, primary alcohol precipitation, rough separation, redissolution, primary filtering decontamination, secondary filtering purification, alcohol precipitation crystallization, drying and smashing. The invention provides an industrial extraction and purification method of fermentation liquor for producing the hyaluronic acid through a streptococcus zooepidemicus fermentation method. The industrial extraction and purification method provided by the invention has the characteristics of short process route and production period, high adaptability, low production cost and good effect, can be concluded into a secondary filtering and secondary alcohol precipitation extraction process and is proved to be feasible through a small-scale test and a pilot scale test in a laboratory and verification in actual production and can meet the requirement for industrialized production.

Description

technical field [0001] The invention relates to the field of microorganisms and biological products, in particular to a production method for extracting and purifying hyaluronic acid. Background technique [0002] Hyaluronic acid, referred to as HA, is an acidic polymer mucopolysaccharide formed by N-acetylglucosamine and D-glucuronic acid linked by β-1,4 glycosidic bonds. It has good hydrophilicity, biocompatibility and moisturizing function, and has been widely used in the fields of cosmetics, food and medicine. The production methods of hyaluronic acid mainly include: extraction method and microbial fermentation method. Among them, the extraction method is restricted by the source of raw materials and cannot be produced on a large scale, while the fermentation method has the characteristics of a wide range of raw material sources, so it has become the mainstream of industrial production of HA. The fermentative production of hyaluronic acid uses Streptococcus zooepidemicu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
Inventor 柯常毅邱平李涛
Owner SICHUAN HENGYI TECH
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