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Method for cultivating cordyceps militaris by using cordyceps militaris strains to infect silkworm larvae

A cultivation method and technology of Cordyceps sinensis, applied in the directions of botanical equipment and methods, application, horticulture, etc., can solve the problems of difficult technical control and complicated operation, and achieve easy large-scale cultivation, simple operation, high infection rate and sloppy rate. Effect

Active Publication Date: 2013-08-28
刘东波
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is more complicated to operate, and the technology is difficult to control

Method used

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  • Method for cultivating cordyceps militaris by using cordyceps militaris strains to infect silkworm larvae
  • Method for cultivating cordyceps militaris by using cordyceps militaris strains to infect silkworm larvae

Examples

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Effect test

Embodiment 1

[0013] (1) Selection of silkworms: choose silkworms on the third day of the fifth instar as hosts;

[0014] (2) Preparation of Cordyceps militaris strains: Divide the rejuvenated Cordyceps militaris slant strains into soybean size, inoculate every 2~3 pieces into a bottle of liquid medium, culture at 23°C and 130r / min for 5 days to ferment liquid, through sterile gauze to obtain liquid strains;

[0015] (3) Preparation of nutrient soil: prepare soil with a water content of 10%, sterilize at 121°C, 0.1 Mpa for 30 min;

[0016] (4) Inoculation: Put the above-mentioned silkworm on the ultra-clean workbench for ultraviolet sterilization for 30 minutes, and then use a disposable syringe to draw 0.2ml of liquid bacteria and inject it into the silkworm;

[0017] (5) Pre-cultivation management: Put the inoculated silkworms into the nutrient soil mentioned above, and place them at 9°C in the dark for 10 days;

[0018] (6) Post-cultivation management: culture the silkworms cultivated ...

Embodiment 2

[0021] (1) Selection of silkworm: choose the silkworm on the 5th day of the fifth instar as the host;

[0022] (2) Preparation of Cordyceps militaris strains: Divide the rejuvenated Cordyceps militaris slant strains into soybean size, inoculate every 2~3 pieces into a bottle of liquid medium, culture at 23°C and 130r / min for 5 days to ferment liquid, through sterile gauze to obtain liquid strains;

[0023] (3) Preparation of nutrient soil: prepare soil with a water content of 15%, and sterilize at 121°C, 0.1 Mpa for 30 minutes;

[0024] (4) Inoculation: Put the above-mentioned silkworm on the ultra-clean workbench for ultraviolet sterilization for 50 minutes, and then use a disposable syringe to draw 0.3ml of liquid bacteria and inject it into the silkworm;

[0025] (5) Pre-culture management: Put the inoculated silkworms into the above-mentioned nutrient soil, and place them at 7°C in the dark for 12 days;

[0026] (6) Post-culture management: culture the silkworm cultured ...

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Abstract

A method for cultivating cordyceps militaris includes steps of (1) silkworm selection: 3rd-5thd silkworms at five years old are selected as hosts; (2) strain producing: cordyceps militaris strains are inoculated in fluid media for shake cultivating, and then liquid strains are obtained after the cordyceps militaris strains are filtered through a sterile gauze for strain ball removing; (3) nutrient soil producing: soil with 5-20% of water content is prepared and serves as nutrient soil after being sterilized at high template and high pressure; (4) inoculating: the silkworms are placed under ultraviolet for sterilizing for 20-50 min, and then 0.2-0.4ml liquid strains are injected to be inoculated into the silkworms; and (5) cultivating management: the inoculated silkworms are placed in the nutrient soil at 5-12 DEG C for light-shielded cultivating for 5-12 d, and then are transferred to be cultivated in the nutrient soil with light intensity to be 50-200 lx, temperature to be 21-23 DEG C, humidity to be 86-89% for 35-60 d to obtain the cordyceps militaris. The method has the advantages of being simple to operate, low in cost, high in infection rate and germination rate, good in stability and the like, is suitable for mass production and has a wide development prospect.

Description

technical field [0001] The invention relates to a method for cultivating Cordyceps militaris by infecting living silkworm larvae with the strain of Cordyceps militaris. Background technique [0002] Cordyceps sinensis is a class of medicinal and edible fungi with significant health functions, the most famous of which is Cordyceps sinensis, and its related products involve health care products, food, Chinese patent medicines and other categories of more than a dozen varieties, enjoying a high reputation all over the world. But the host of Cordyceps sinensis is specific, the living environment is harsh, and artificial cultivation is difficult to be completely successful. Moreover, due to the long life cycle of its natural host bat moth, many natural enemies, and many diseases, the output of naturally reproduced larvae is seriously insufficient. Seriously, artificial premature and excessive harvesting and digging and desertification of meadows have greatly reduced the chances o...

Claims

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Application Information

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IPC IPC(8): A01G1/04A01K67/04
Inventor 刘东波颜新培王蕾李坚赖锡湖
Owner 刘东波
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