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Specificity method for detecting candida albicans in clinical specimen

A Candida albicans, specific technology, applied in the field of clinical medical testing, can solve the problems of many influencing factors, long time required, troublesome operation, etc., and achieve the effect of high sensitivity, long storage time and short time

Active Publication Date: 2013-07-10
众爱生河北生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Analyzing some of the above diagnostic methods, although these methods currently play a dominant role in the monitoring, prevention and control

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0019] Embodiment 1. Clinical detection of Candida albicans in female vagina

[0020] Preparation of latex sensitization and colored antibody reagents, 1. Preparation of antiserum, immunization of animals with a mixed bacterial solution containing multiple strains of Candida to obtain immune serum, and crude antibody products obtained from immunized animals through precipitation, centrifugation, column passing, etc. Chemical method to remove impurities to obtain specific antibodies to Candida albicans; 2. Latex labeling and coloring treatment to adsorb specific antibodies to polystyrene latex particles to obtain sensitized polystyrene latex particles, and then use crystal violet dye Make it colored at 4° C., and turn into purple; 3. Preservative treatment, add preservative to the colored antibody-sensitized latex particle liquid, and prepare a stable latex-sensitized colored antibody reagent.

[0021] Specimen detection stage, specifically including the collection of vaginal s...

Example Embodiment

[0022] Example 2. Detection of samples such as other cultures

[0023] Latex-sensitized colored antibody reagents were prepared as described in Example 1.

[0024] In the stage of specimen detection, take the culture specimen of Candida albicans in the laboratory, dip the culture with a sterile swab, and mix it directly on the reaction card containing 100ul latex sensitization and coloring antibody, mix it gently, and place it for 5 ~10 minutes, shake the reaction card by hand (or place it on a horizontal shaker), and observe that there are obvious blue agglutinated particles.

[0025] Using the present invention to detect Candida in vaginal secretions and other specimens does not require any detection equipment and equipment, the entire detection method is simple and sensitive, the result is easy to judge, and the detection process only takes 5 to 10 minutes.

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PUM

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Abstract

The invention discloses a specificity method for detecting candida albicans in a clinical specimen. The specificity method comprises the following process steps of preparing and purifying antiserum, marking and coloring by using emulsion, carrying out preservative treatment, detecting the candida albicans and the like. Compared with the prior art, the specificity method has the advantages of rapidness, simplicity and convenience, high sensitivity, easiness in result judgment and the like.

Description

[0001] technical field [0002] The invention belongs to the field of clinical medical examination, and is a specific method for detecting Candida albicans in clinical specimens. [0003] Background technique [0004] Candidiasis refers to primary or secondary infection caused by Candida albicans (Candida albicans). The skin and mucous membranes of human reproductive organs are infected by Candida albicans and clinical symptoms are called genital candidiasis, including candidal vaginitis and balanitis, the most common being female vaginitis. The diagnosis of candidal vaginitis is based on the patient's clinical symptoms, doctor's physical examination and laboratory test results. [0005] The current clinical application of Candida detection methods are as follows: [0006] Conventional smear microscopy is the most basic bacteriological examination method. Its advantage is that it is simple, fast and cheap, and the result can be obtained on the same day; the disadvantage ...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N33/531
Inventor 文凤枝
Owner 众爱生河北生物科技有限公司
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