Cattle food-and-mouth disease virus A type synthetic peptide and preparation and application thereof

Abstract

The invention discloses a cattle food-and-mouth disease virus A type synthetic peptide. The cattle food-and-mouth disease virus A type synthetic peptide has the following amino acid sequence: acetyl-YDLDFEALKPHFKSLGQTITPADKSPPSVYNGTCKYSAPATRRGDLGSLAARLAACLPASFNYGAIRAT-amide. The cattle food-and-mouth disease virus A type synthetic peptide can be used as an effective novel vaccine for the A type food-and-mouth disease in production practice; simultaneously, the realization of the test provides a certain foundation for further perfecting the construction of a food-and-mouth disease virus A type synthetic peptide vaccine and the construction of other food-and-mouth disease subtype synthetic peptide vaccines; and the new ideal proposed in the research of the synthetic peptide vaccine and the construction of a new method of the synthetic peptide vaccine provide theoretical foundation and technical support for further perfecting the development and research of the multiple antigenic peptide, multivalent peptides and joint peptide vaccines in future.

Description

technical field [0001] The invention relates to a synthetic peptide, in particular to a bovine foot-and-mouth disease virus type A synthetic peptide and its preparation and application. Background technique [0002] Foot-and-mouth disease (FMD) is an acute, febrile, highly contagious and rapid long-distance transmission caused by foot-and-mouth disease virus (FMDV) infection. severe infectious disease. The outbreak of this disease has caused great losses to animal husbandry production and import and export trade. Therefore, the Food and Agriculture Organization of the United Nations and the International Office of Epizootics have listed it as the number one infectious disease that must be reported. [0003] Since Loeffer discovered the disease in 1879, people have made unremitting efforts to control the disease. Vaccination is a reliable and effective means of specific prevention of FMD, and a safe and effective vaccine is a prerequisite for the successful prevention, cont...

AI Technical Summary

Problems solved by technology

[0007] Although significant progress has been made in the field of synthetic peptide vaccines, there are still many problems to be solved in practical application: 1. Applying to the research of FMD synthetic peptide vaccines The antigenic epitopes are too limited, most of which are concentrated in the 140-160 and 200-213 amino acids of the structural protein VP1, and the antigenic epitopes to choose from are poor.

2. The immunogenicity of synthetic peptides is low, which requires the selected epitope itself to have strong immunogenicity

3. The conformation of the antigenic peptide must be consistent with the conformation of the antigenic determinant on the intact virus, but various methods such as prediction and simulation currently applied cannot grasp its natural composition, and the existing synthesis technology can only synthesize straight chain peptides , its three-dimensional space structure is not the same as that of natural molecules

4. The mechanism of action between the selected carrier and adjuvant and the antigenic peptide and the body is not clear enough

Affect the full play of the efficacy of the carrier and adjuvant, and cannot more reliably improve the immune efficacy and protective effect of the synthetic peptide vaccine

5. The limitations of peptide synthesis and purification technology and the application of various adjuvants increase the toxic and side effects of synthetic peptide vaccines to varying degrees, etc.

However, there is still little research on synthetic peptide vaccines for bovine foot-and-mouth disease type A

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