Method for separating and purifying various components from cefaclor enzymatic reaction solution

Abstract

The invention relates to a method for separating and purifying various components from a cefaclor enzymatic reaction solution, and the method comprises the following steps of: (1) recovering an unreacted mother nucleus 7-ACCA from the cefaclor enzymatic reaction solution by adopting an acid precipitation method; (2) separating and purifying cefaclor by adopting macroporous adsorption resin; and (3) separating and purifying D-phenylglycine by adopting cation exchange resin. The method disclosed by the invention can be used for separating and purifying the unreacted mother nucleus 7-ACCA, the product cefaclor and the by-product D-phenylglycine from the reaction solution used for enzymatically synthesizing the cefaclor, sufficiently utilizes the raw materials and reduces the production cost; and products obtained by adopting the method disclosed by the invention are high in yield purity and suitable for popularization and usage.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for separating and purifying components from cefaclor enzymatic reaction liquid. Background technique [0002] Cefaclor (Cefaclor) is a semi-synthetic second-generation cephalosporin antibiotic, which has good antibacterial activity against Gram-positive bacteria, high efficiency, broad spectrum, and good chemical stability. One of the main drugs for bacterial infections. Since its launch in the 1970s, cefaclor has been highly praised as an antibiotic with high efficiency, broad spectrum and good clinical safety. Its chemical structural formula is as follows: [0003] ; [0004] In industry, cefaclor is mainly prepared by condensation of 7-amino-3-chloro-deacetoxy-cephalosporanic acid (7-ACCA) and activated D-phenylglycine. There are two processes of chemical condensation and enzymatic condensation. Patent WO2006 / 069984 discloses a method for enzymatic condensation of ce...

AI Technical Summary

Problems solved by technology

The resulting aqueous mixture contains cefaclor in an amount >10 (w / w), 7-ACCA in an amount <2 (w / w), and D- Phenylglycine, this patent also discloses the separation and purification method of cefaclor, the disadvantage of this patent is that the separation of unreacted 7-ACCA and by-product D-phenylglycine has not been studied

Patent CN 200810038896.7 discloses a method for purifying cefaclor from cefaclor and naphthol complex, the method is mainly to use organic solvent to selectively extract the naphthol in the compound of cefaclor and naphthol, and then collect and obtain The aqueous phase is adsorbed by a macroporous adsorption resin for the separation and purification of cefaclor. The disadvantage of this patent is that the complexing agent naphthol is added to the enzymatic reaction solution, and then the compound of cefaclor and naphthol is obtained. , and then used for further separation and purification. This process increases the steps of separation and purification. In addition, the eluent used in this process is a mixture of methanol-water, isopropanol-water, etc. These eluents pollute the environment and are harmful to the operation. health of personnel is at risk

The disadvantage of this process is that it needs to enzymatically hydrolyze the antibiotics that have been synthesized, which reduces the actual yield of a single batch of antibiotics, and the recovery and reuse process of the mother nucleus is complicated.

At present, there is no report on the separation and purification of D-phenylglycine in the reaction solution of enzymatic condensation of cefaclor, and D-phenylglycine has a large content in the enzymatic reaction solution. If it can be recycled and reused, it will be able to Further reduce the cost of enzymatic synthesis of cefaclor