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Application of superparamagnetic iron oxide nanoparticle applied in transdermal drug delivery system

A technology of iron oxide nanoparticles and superparamagnetism, which is applied in the direction of medical preparations with non-active ingredients, medical preparations containing active ingredients, and pharmaceutical formulas, to achieve the effect of overcoming the single administration method and non-toxic skin

Active Publication Date: 2014-11-19
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the application of superparamagnetic iron oxide nanoparticles in transdermal drug delivery. If the method of TDDS can be used for transdermal drug loading of SPION, it will be able to effectively solve the problem of SPION's existing drug delivery methods and magnetic fields. Implemented restrictions

Method used

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  • Application of superparamagnetic iron oxide nanoparticle applied in transdermal drug delivery system
  • Application of superparamagnetic iron oxide nanoparticle applied in transdermal drug delivery system
  • Application of superparamagnetic iron oxide nanoparticle applied in transdermal drug delivery system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Co-precipitation method to synthesize superparamagnetic iron oxide nanoparticles

[0019] Weigh 10mg ferric chloride (FeCl 2 4H 2 O) and 28mg sodium metasilicate (Na 2 SiO 3 9H 2 O) Dissolve in 30ml of degassed ultrapure water to obtain a mixed iron salt solution, adjust the pH value to 3.0 with hydrochloric acid solution, raise the temperature to 30°C, add lye drop by drop until the pH value of the reaction solution is 9-10 , stop adding lye, and then heat in a water bath, mature the reaction suspension at 80°C for 1 hour, centrifuge at 8000rpm for 5 minutes, separate the precipitate, wash the precipitate three times with ultrapure water and absolute ethanol, and place it at 70 °C, vacuum-dried and set aside.

[0020] (2) Surface amino modification

[0021] Ultrasonically disperse the precipitate obtained in step (1) in 100ml of boric acid solution with a pH of 5.0, add 2mg of carbodiimide for catalysis and 2ml of triethoxysilane, 80°C water bath, magnetic st...

Embodiment 2

[0025] (1) Magnetic fluid particle size and potential measurement

[0026] Use a 200-mesh copper mesh support film dedicated to transmission electron microscopy to dip an appropriate amount of the Epi-SPION suspension prepared in Example 1, dry it at room temperature, and observe the size and shape of the nanoparticles under a JEM-1200EX transmission electron microscope. For the results, see figure 1 , the particle size of Epi-SPION nanoparticles is 8-10nm, the particles are regular, approximately spherical, the dispersion is good, and no obvious agglomeration occurs; take another appropriate amount (about 0.5ml) of nanoparticle suspension, ultrasonic After dispersion treatment, dilute to 3-5 times, and use Zetasizer Nano S90 high-sensitivity nanometer particle size analyzer to measure the particle size and Zeta potential of nanoparticles. For the measurement results, see figure 1 : The particle size of EPI-SPION is mainly distributed at 28.1nm, showing a normal distribution, ...

Embodiment 3

[0030] (1) Cell culture

[0031] HaCaT cells (normal human skin cell line, KGI Biotech) were revived and incubated with RPM1640 medium containing 10% fetal bovine serum at 37°C, 5% CO 2 cultured in an environment with EDTA-trypsinization and subcultured every other day.

[0032] (2) Cytotoxicity study

[0033]The above-mentioned cells were subcultured to reach 80-90% confluence, digested with 0.25% trypsin-EDTA solution, and prepared into a single-cell suspension with RPM1640 culture medium containing 10% fetal bovine serum. 3 Inoculate / well in a 96-well plate; at 37°C, 5% CO 2 Cultivate under conditions for 24 hours; discard the supernatant, wash twice with PBS solution, add EPI-SPION, SPION-containing media in turn, the concentrations of nanoparticles in the media are 50 μg / ml, 100 μg / ml, 200 μg / ml, respectively. ml, 400μg / ml, 600μg / ml, 800μg / ml, 1000μg / ml of the above nanoparticle suspension and 10% fetal bovine serum culture solution 200μl, design 3 replicate wells for ...

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Abstract

The invention provides application of a superparamagnetic iron oxide nanoparticle applied in a transdermal drug delivery system. The superparamagnetic iron oxide nanoparticle is taken as a drug carrier of epirubicin; an epirubicin-superparamagnetic iron oxide nanoparticle transdermal drug delivery system is prepared; and a primary amine group (-NH2) exists on the surface of the superparamagnetic iron oxide nanoparticle. The superparamagnetic iron oxide nanoparticle can achieve transdermal absorption and has no poison, stimulation or anaphylactic reaction to skins, and the defects that a superparamagnetic iron oxide nanoparticle is single in drug-delivery manner and the magnetic field implementation is limited are overcome.

Description

technical field [0001] The invention belongs to the field of pharmacy and relates to the application of superparamagnetic iron oxide nanoparticles in the preparation of a transdermal drug delivery system. Background technique [0002] Superparamagnetic Iron Oxide Nanoparticles (SPION) refers to nanoscale particles with magnetic responsiveness. When the particle size of magnetic nanoparticles is smaller than its superparamagnetic critical size, the particles enter the superparamagnetic state. It is smaller or close to the size of cells, viruses, proteins, genes, etc., which is conducive to the interaction between them, and the coverage of biomolecules on their surfaces is also easier to achieve. Magnetic nanoparticles can move directional and fast under the action of an external magnetic field, which can further shorten the time for drug directional enrichment, and under the action of an alternating magnetic field, it can produce a thermal effect, which can better control the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/04A61K41/00A61K31/704A61K9/14
Inventor 卢晓阳饶跃峰洪东升
Owner ZHEJIANG UNIV
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