A method for detecting the sensitivity of brown planthopper to pesticides

Abstract

The invention discloses a method for detecting sensibility of brown planthopper to medicament. The method comprises following steps: A, medicament preparation: nitenpyram original medicine is dissolved by acetone to be prepared into pesticide mother solution, and the mother solution is dissolved by acetone to be prepared into liquor; B, a certain amount of liquor is absorbed to be placed into a glass bottle to be rolled evenly, susceptibility determination of liquor is performed after the acetone is evaporated fully, comparison medicament is processed by acetone, and comparison is repeated for 3 to 4 times; C, third instar nymphs of brown planthopper are placed into the prepared glass bottles with medicine films, death rate is checked after 90 min, and the brown planthopper which can not move is determined to be dead; D, the death rate of a matched group is smaller than 5%, which proves that an experiment is effective; E, the sensibility of brown planthopper to medicament is judged as per the adjusted mortality ratio after 90 min of the brown planthopper in the glass bottles with medicine films, in other words, if the adjusted mortality ratio is larger than 90%, which shows that the brown planthopper is sensitive to the medicament. The method is simple, easy to implement, and convenient to operate, solves the problems of complex operations and large workload of an impregnation method and a drop method of rice stem, and can finish detection in field quickly.

Description

technical field [0001] The invention belongs to the field of plant protection and pesticide science, and more specifically relates to a method for rapidly detecting the sensitivity of brown planthoppers to chemicals, which is suitable for a rapid detection of the sensitivity of brown planthoppers to nitenpyram. Background technique [0002] In China's agricultural production, the brown planthopper is very harmful to rice, and it is the primary pest in rice production in China and many Asian countries. When the brown planthopper occurs in large numbers, it will cause the reduction or even extinction of rice production. For many years, the control of brown planthopper in many rice planting areas has mainly relied on chemical pesticides, and many areas have generally reported that the control effect of insecticides commonly used in production on brown planthopper is declining. Extensive and long-term use of insecticides reduces the sensitivity of pests to insecticides and even ...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for quickly detecting the sensitivity of rice field brown planthoppers to commonly used chemicals. The method is easy to implement and easy to operate. 50 For problems such as long period of time, the detection can be completed quickly in the field

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