Preparation method of medical grade temperature sensitive chitosan blocking agent used for preventing leakage of cerebrospinal fluid

Abstract

The invention relates to the medical biomaterial technical field. The current product which is widely used for preventing postoperative leakage of cerebrospinal fluid has the disadvantages of tedious operation step, expanded volume and latent infection risk. The invention provides a preparation method for medical grade temperature sensitive hydroxybutyl chitosan capable of internally using, a purifying method and an application method. The prepared material has appropriate deacetylation degree, can guarantee good biocompatibility, and has the advantages of simple operation, complete wound enclosing, injectable performance, biodegradability and excellent plugging effect. According to the invention, a chitosan raw material with deacetylation degree of 10-70% is prepared, then a hydroxyl butylation reaction is carried out, and a unique temperature sensitive performance is endowed for chitosan; and then a unique salting out method and a phase transition method are employed for purifying. The prepared medical grade temperature sensitive chitosan preparation has good temperature sensitive performance, good gel resilience performance, simple usage; and good biocompatibility and biological mechanical property can prove that the preparation method of the medical grade temperature sensitive chitosan blocking agent used for preventing leakage of cerebrospinal fluid has good clinical application prospect.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, in particular to a medical-grade temperature-sensitive chitosan preparation and its preparation method and application. The preparation has good biocompatibility and temperature responsiveness and can be used as a preparation for preventing cerebrospinal fluid leakage to use. Background technique [0002] Chitosan is a naturally occurring ecological alkaline polysaccharide. Years of research have shown that chitosan has very good biological properties and a variety of specific biological functions, such as antibacterial, anti-adhesion, wound healing, etc., because chitosan molecules It contains relatively active 2-NH2 and 6-OH, so a variety of chemical modifications can be carried out to give it more chemical properties and biological functions. For example, the hydroxybutyl chitosan used in this product is chitosan Results of sugar hydroxybutylation. As we all know, the amino grou...

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Problems solved by technology

Among them, EVICEL and Tisseel are blood products, and there is a possibility of virus infection during use; the safety of BioGlue is still in doubt; and DuraSeal is a synthetic pure chemical material, which is mainly obtained by mixing the two materials Colloidal occluding substances, although their use has been developed and no longer required after suturing the dura mater, but its use is limited due to the large volume expansion of the material itself in the body fluid environment

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