Single-cell clamping method and single-cell position regulating device

A regulating device, single-cell technology, applied in biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc., to achieve high-precision and precise positioning, improve versatility, and improve the effect of success rate

Inactive Publication Date: 2013-06-05
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] During cell micromanipulation, the positioning operation of cells is currently completed by suction and exhalation of the suction tube. It is relatively easy to use negative pressure to absorb cells, but it is a direct contact operation
The direct contact method will damage the cells and affect the success rate of the experiment

Method used

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Embodiment Construction

[0022] The specific implementation of the present invention will be further introduced below in conjunction with the accompanying drawings, but the implementation and protection scope of the present invention are not limited thereto.

[0023] Such as figure 1 , a single-cell position adjustment device, including two cell holding tubes (1 and 2), a three-degree-of-freedom micro-motion platform for separately controlling the telescopic movement of each cell holding tube and providing the cell holding tube with the required The micropump unit 7 of the flow rate; one end of each cell holding tube is respectively connected to the micropump unit 7 through soft microtubes (5 and 6), wherein the two cell holding tubes are located on the same straight line, and distribution on both sides of the cells. The two cell holding tubes (1 and 2) are fixedly installed on two three-degree-of-freedom micro-motion platforms (3 and 4), and the two three-degree-of-freedom micro-motion platforms (K...

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Abstract

The invention provides a single-cell clamping method and a single-cell position regulating device. The method is that in the regulating operation process of cells, fluid pressure and fluid viscosity force between clamping tubes are used for clamping the cells, the cells are wrapped and clamped to move in a non-contacting mode through the movement of the clamping tubes, and so that the position regulation of the cells is achieved. The device comprises two cell clamping tubes, two soft micro tubes, a control unit and a micropump unit which supplies the needed flow to the cell clamping tubes. Each cell clamping tube is connected with the micropump unit through the soft micro tubes, and the two cell clamping tubes are installed on the control unit and are distributed on two sides of the cells. The single-cell clamping method and the single-cell position regulating device can be suitable for the cells with different shapes and different sizes, the three-dimensional position regulation of the cells is achieved, mechanical damage to the cells can be avoided, and the single-cell clamping method and the single-cell position regulating device are beneficial to improving the success rate of cell experiment operation.

Description

technical field [0001] The invention relates to the technical field of single cell position adjustment, in particular to a single cell clamping method and a position adjustment device thereof. Background technique [0002] At present, cell micromanipulation in bioengineering is basically in a manual or semi-automatic state, and there are problems such as low efficiency, low precision, low repeatability, high cost, and high labor intensity, which greatly restrict the popularization and application of micromanipulation technology . In addition, it takes 2 years to train a skilled operator. Researchers generally hope to replace manual work with machinery, and replace manual work with automation, so that micromanipulation technology can be simplified and automated, and then engineering can be realized. [0003] In the process of cell micromanipulation, the positioning operation of cells is currently completed by suction and exhalation of the suction tube. It is relatively eas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00C12M1/36C12M1/00
Inventor 黄维军雷武臣张勤
Owner SOUTH CHINA UNIV OF TECH
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