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A method for extracting oleuropein from Qiaoling flower

An oleuropein and extraction technology, which is applied in the field of extracting high-purity oleuropein, can solve the problem of low oleuropein content, and achieve the effects of improving separation and purification efficiency, high yield, and convenient industrialized operation.

Inactive Publication Date: 2015-09-23
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problem in the prior art that the content of oleuropein enriched from Qiaoling flower is not high, the present invention provides a method for extracting high-purity oleuropein from Qiaoling flower

Method used

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  • A method for extracting oleuropein from Qiaoling flower
  • A method for extracting oleuropein from Qiaoling flower
  • A method for extracting oleuropein from Qiaoling flower

Examples

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Embodiment 1

[0039] A method for extracting oleuropein from Qiaolinghua, comprising the steps of soaking crushed dried Qiaolinghua in ethanol and then ultrasonically extracting, concentrating the extract into an extract, and purifying and separating the obtained extract ;

[0040] The method of ultrasonically extracting and concentrating Qiaolinghua after soaking is as follows: soak 1 kg of dried Qiaolinghua with a mass of 12 kg and 90% volume concentration of ethanol for 36 hours, and then ultrasonically extract 50% at 60°C. Minutes, filtered, repeated extraction of the filter residue 3 times, combined extracts, concentrated under reduced pressure distillation at 50-60°C, to obtain 210g of extract;

[0041] The step of described purification separation is:

[0042] Step 1: Dissolve 200g of the prepared extract with 7 times the mass of methanol, filter, mix the filtrate with silica gel-diatomaceous earth (mass ratio 1:1) with a mass multiple of 4 times the mass, evaporate the solvent and ...

Embodiment 2

[0046] A method for extracting oleuropein from Qiaolinghua, comprising the steps of soaking crushed dried Qiaolinghua in ethanol and then ultrasonically extracting, concentrating the extract into an extract, and purifying and separating the obtained extract ;

[0047] The method of ultrasonically extracting and concentrating Qiaolinghua after soaking is as follows: soak 1 kg of dried Qiaolinghua with a mass of 10 kilograms and 90% volume concentration of ethanol for 24 hours, and then ultrasonically extract it at 50°C for 30 Minutes, filtered, repeated extraction of the filter residue twice, combined extracts, concentrated under reduced pressure at 50-60°C to obtain 205 g of extract;

[0048] The step of described purification separation is:

[0049] Step 1: Dissolve the prepared 200g extract with 7 times the mass of acetonitrile, filter, mix the filtrate with silica gel-diatomaceous earth (mass ratio 2:1) with a mass multiple of 4 times the mass, evaporate the solvent and th...

Embodiment 3

[0053] A method for extracting oleuropein from Qiaolinghua, comprising the steps of soaking crushed dried Qiaolinghua in ethanol and then ultrasonically extracting, concentrating the extract into an extract, and purifying and separating the obtained extract ;

[0054] The method of ultrasonically extracting and concentrating Qiaolinghua after soaking is as follows: 3 kg of crushed dried Qiaolinghua is soaked in 30 kg of ethanol with a volume concentration of 90% for 24 hours, and then ultrasonically extracted at 60°C for 30 Minutes, filtered, repeated extraction of the filter residue 3 times, combined extracts, concentrated under reduced pressure distillation at 50-60°C, to obtain 708g of extract;

[0055] The step of described purification separation is:

[0056] Step 1: Dissolve the prepared 700g extract with 5 times the mass of methanol, filter, mix the filtrate with silica gel-diatomaceous earth (mass ratio 1:2) with a mass multiple of 4 times the mass, and evaporate the ...

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Abstract

The invention belongs to the technical field of extracting and separating of traditional Chinese medicine active ingredients and in particular relates to a method for extracting oleuropein from syringa pubescens. The method comprises the following steps of: soaking the crushed dry syringa pubescens in alcohol and ultrasonically extracting the soaked syringa pubescens, and concentrating the extracting solution into extract; and purifying and separating the prepared extract, i.e., preparing high-purity secoiridiod glycoside compound-oleuropein by the processes such as ultrasound-assisted organic solvent alcohol extractiion, dissolving and filtering, solid-phase extraction, reversed-phase flash column chromatography, active carbon adsorption, re-crystallization and the like. The method provided by the invention is simple and quick; and the obtained product is high in purity and high in yield, so that a foundation is laid for the future development and research of oleuropein.

Description

technical field [0001] The invention belongs to the technical field of extracting and separating effective components of traditional Chinese medicines, and in particular relates to a method for extracting high-purity oleuropein from Qiaolinghua. Background technique [0002] Qiao Linghua ( Syringa pubescens Turca) is a plant of the genus Clove in the family Oleaceae, a shrub, also known as hair (leaf) clove, four seasons clove, small-leaved clove, produced in Henan, Hebei, Shaanxi and other places. It has been found through research that Qiaolinghua contains triterpenoids, flavonoids and saponins, and its content of oleuropein is relatively high, which has great research and development value. [0003] Oleuropein is a secoiridoid glycoside compound found in at least 25 species of Oleaceae. Oleuropein is another compound with strong biological activity. It has been reported in the literature that it has significant antifungal, anti-inflammatory, antiviral, antioxidativ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/04C07H1/08
Inventor 邓瑞雪刘普尹卫平高嘉屿王怡冉牛亚琪段文录时清亮柴元武
Owner HENAN UNIV OF SCI & TECH
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