Deodorized fermentation liquor of excrements of livestocks and preparation method thereof
A technology of livestock and poultry manure and fermentation liquid, which is applied in the preparation of organic fertilizers, organic fertilizers, waste fuels, etc., can solve technical difficulties, it is not easy to discover or create functional microorganisms, and the overall benefits are not obvious, etc., to achieve good decomposition effect, quality improvement effect
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Embodiment 1
[0028] 1. Activation medium for anaerobic cellulolytic bacteria:
[0029] Activation medium for anaerobic cellulolytic bacteria: including the following components: peptone 8g, beef extract 4g, sodium chloride 5g, potassium phosphate 5g; distilled water is quantified to 1000ML.
[0030] The preparation method of the activated medium is to mix the components in proportion, adjust the pH value to 7.1-7.5 after dissolving, sterilize at 121° C. for 15 minutes, and then pack them into sterilized flasks. Inoculate the aerobic cellulolytic bacteria into the culture medium to obtain the anaerobic cellulolytic bacteria seed solution.
[0031] 2. Inoculate Lactobacillus faecalis into the activation medium:
[0032] Peptone (Proteose peptone) 8g,
[0033] Beef extract (Beet extract) 8g,
[0034] Yeast extract (Yeast extract) 5g,
[0035] Glucose (Dextrose) 20g,
[0036] Sodium acetate (Sodium acetate) 5g,
[0037] Manganese sulfate (Manganese sulfate) 0.03g,
[0038] Potassium pho...
Embodiment 2
[0061] 1. Activation medium for anaerobic cellulolytic bacteria:
[0062] Activation medium for anaerobic cellulolytic bacteria: including the following components: 10 g of peptone, 4 g of beef extract, 5 g of sodium chloride, and 6 g of potassium phosphate.
[0063] The preparation method of the activated medium is to mix the components in proportion, adjust the pH value to 7.1-7.5 after dissolving, sterilize at 121° C. for 15 minutes, and then pack them into sterilized flasks. Inoculate the aerobic cellulolytic bacteria into the culture medium to obtain the anaerobic cellulolytic bacteria seed solution.
[0064] 2. Inoculate Lactobacillus faecalis into the activation medium:
[0065] Peptone (Proteose peptone) 10g,
[0066] Beef extract (Beet extract) 10g,
[0067] Yeast extract (Yeast extract) 5g,
[0068] Glucose (Dextrose) 20g,
[0069] Sodium acetate (Sodium acetate) 5g,
Embodiment 3
[0094] 1. Activation medium for anaerobic cellulolytic bacteria:
[0095] Activation medium for anaerobic cellulolytic bacteria: including the following components: peptone 9g, beef extract 5g, sodium chloride 6g, potassium phosphate 6g; distilled water is quantified to 1000ML.
[0096] The preparation method of the activated medium is to mix the components in proportion, adjust the pH value to 7.1-7.5 after dissolving, sterilize at 121° C. for 15 minutes, and then pack them into sterilized flasks. Inoculate the aerobic cellulolytic bacteria into the culture medium to obtain the anaerobic cellulolytic bacteria seed solution.
[0097] 2. Inoculate Lactobacillus faecalis into the activation medium:
[0098] Peptone 10g,
[0099] Beef extract 10g,
[0100] Yeast extract 6g,
[0101] Glucose 25g,
[0102] Sodium acetate 5g,
[0103] Manganese sulfate 0.05g,
[0104] Potassium phosphate 3g,
[0105] Distilled water 100ml,
[0106] After stirring and dissolving the above ma...
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