Method for producing R-alpha-hydroxybutyrate by using 1, 2-butanediol as substrate
A technology of hydroxybutyric acid and butanediol, which is applied in the field of producing R-alpha-hydroxybutyric acid, can solve the problems that the method for producing R-alpha-hydroxybutyric acid has not been reported, and achieves easy separation and purification, simple reaction system, Inexpensive effect
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Embodiment 1
[0042] (1) Preparation of biocatalyst
[0043] Gluconobacter oxidans DSM 2003 (purchased from the German Collection of Microorganisms) was selected, cultured by conventional methods, the bacteria were separated and collected, and the bacteria were washed with pH 7.4 potassium phosphate buffer for 3 times, and the bacteria were suspended in deionized water to make the bacteria concentration When reaching 200 g wet cells / liter, the obtained complete cell suspension is the biocatalyst, stored at 4°C for later use;
[0044] Among them, the above-mentioned Gluconobacter oxydans is cultivated using the following medium formula: D-sorbitol 80 g / L; yeast powder 24 g / L; ammonium sulfate 5 g / L; potassium dihydrogen phosphate 2 g / L; magnesium sulfate 5 g / L Liter; Calcium carbonate 10 g / liter.
[0045] (2) Conversion
[0046] The biocatalyst prepared in step (1), ie, intact cells, is mixed with the substrate 1,2-butanediol aqueous solution, so that the concentration of the biocatalyst in the mix...
Embodiment 2
[0052] (1) Preparation of biocatalyst
[0053] Select Gluconobacter oxydans DSM 2003, culture by conventional methods, separate and collect the bacteria, wash the bacteria 3 times with pH 7.4 potassium phosphate buffer, and suspend the bacteria in deionized water to make the bacteria concentration reach 200 g wet cells / liter. The obtained complete cell suspension is the biocatalyst, stored at 4°C for later use;
[0054] Among them, the above-mentioned Gluconobacter oxydans is cultivated using the following medium formula: D-sorbitol 80 g / L; yeast powder 24 g / L; ammonium sulfate 5 g / L; potassium dihydrogen phosphate 2 g / L; magnesium sulfate 5 g / L Liter; Calcium carbonate 10 g / liter.
[0055] (2) Conversion
[0056] Mix the biocatalyst prepared in step (1), i.e. intact cells, with the substrate 1,2-butanediol aqueous solution, so that the concentration of the biocatalyst in the mixture is 100 g wet cells / liter, 1,2-butanediol The concentration is 50 g / L, and disodium edetate is added ...
Embodiment 3
[0062] (1) Preparation of biocatalyst
[0063] Select Gluconobacter oxydans DSM 2003, culture by conventional methods, separate and collect the bacteria, wash the bacteria 3 times with pH 7.4 potassium phosphate buffer, and suspend the bacteria in deionized water to make the bacteria concentration reach 200 g wet cells / liter. The obtained complete cell suspension is the biocatalyst, stored at 4°C for later use;
[0064] Among them, the above-mentioned Gluconobacter oxydans is cultivated using the following medium formula: D-sorbitol 80 g / L; yeast powder 24 g / L; ammonium sulfate 5 g / L; potassium dihydrogen phosphate 2 g / L; magnesium sulfate 5 g / L Liter; Calcium carbonate 10 g / liter.
[0065] (2) Conversion
[0066] The biocatalyst prepared in step (1), ie, intact cells, is mixed with the substrate 1,2-butanediol aqueous solution, so that the concentration of the biocatalyst in the mixture is 120 g wet cells / liter, 1,2-butanediol The concentration is 60 g / L, and disodium edetate is ad...
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