A method for isolating and culturing human meningioma cells
A technology for meningioma and human brain, applied in the field of biomedicine, can solve the problems of inability to obtain a large number of living cells, slow cell growth, and slow cell growth rate.
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[0042] 1 Materials and methods:
[0043] 1.1 Reagents are all cell culture grade reagents, unless otherwise specified are GIBCO products.
[0044] 1.2 The materials come from 6 isolated meningioma tissues excised from neurosurgery patients with meningioma in Huashan Hospital Affiliated to Fudan University.
[0045] 1.3 Method
[0046] (1) Separation of primary human meningioma cells:
[0047] a. The isolated human meningioma tissue was aseptically collected, and the surgically resected meningioma tissue was washed more than 5 times in ice-cold HBSS.
[0048] b. Cut the tissue with scissors, incubate the tissue in PBS containing Dispase / Collegnase and 0.04% DNase at 37°C for 30 minutes, blow and suck more than ten times with a Pasteur pipette, then vertically rest the centrifuge tube for 5 minutes, and collect the upper layer Digest the suspension and collect the digested cells by centrifugation;
[0049] c. Add an appropriate amount of enzyme to the undigested tissue in th...
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