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Method and device for identifying red blood cells infected by malaria parasites

A technology for plasmodium infection and red blood cells, which is applied in the medical field, can solve problems such as low efficiency and poor recognition accuracy of red blood cells, and achieve the effects of fast inspection, high recognition accuracy, and improved recognition accuracy

Active Publication Date: 2016-08-17
SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the embodiments of the present invention is to provide a method for identifying red blood cells infected by malaria parasites, aiming to solve the problems of poor recognition accuracy and low efficiency of red blood cells infected by malaria parasites in existing technical solutions

Method used

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  • Method and device for identifying red blood cells infected by malaria parasites
  • Method and device for identifying red blood cells infected by malaria parasites
  • Method and device for identifying red blood cells infected by malaria parasites

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Embodiment 1

[0097] This embodiment provides a method for identifying red blood cells infected by Plasmodium. The specific technical scenario implemented in this embodiment can be as follows: the method provided in this embodiment is completed by a flow cytometer, and the analyzer can specifically adopt Shenzhen Mindray Biomedical Technology Co., Ltd. The BC series flow cytometer produced by Electronics Co., Ltd., the specific structure of the analyzer is as follows: Figure 7 As shown, the formula of the hemolytic agent can be: reagent A, and the reagent A can specifically include: dye A (0.5ppm), decyl isoquinoline bromide (0.4g / L), polyoxyethylene lauryl alcohol (23) Ether (1.3g / L), sodium benzoate (2.0g / L), methanol (50g / L), sodium dihydrogen phosphate (3g / L) and disodium hydrogen phosphate (4.8g / L). Wherein the structural formula of dye A is as follows:

[0098]

[0099] In addition, the volume of the above-mentioned reagents can be 1 ml, the above-mentioned blood sample can be 20...

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Abstract

The present invention is applicable to the medical field, and provides a method and device for identifying red blood cells infected by Plasmodium, the method comprising: acquiring forward scattered light signals and side scattered light signals of cells in a sample, and optional fluorescent signals; Obtain a first two-dimensional scatter diagram according to the forward scattered light signal and side scattered light signal, or obtain a three-dimensional scatter diagram based on the forward scattered light signal, side scattered light signal and fluorescence signal; The first two-dimensional scattergram or the three-dimensional scattergram shows cells identified as Plasmodium-infected red blood cells within a predetermined region. The technical solution provided by the invention has the advantage of high recognition accuracy.

Description

technical field [0001] The invention belongs to the medical field, and in particular relates to a technology for identifying red blood cells infected by malaria parasites. Background technique [0002] Plasmodium infection is a common infectious disease in many parts of the world, especially in Africa. Plasmodium enters the human blood circulation system through mosquito bites, and reproduces and proliferates in red blood cells. Malaria often causes clinical symptoms such as fever and chills, and if not treated in time, it may even lead to death. Malaria is an infectious disease that seriously threatens human health. [0003] The current diagnosis of malaria requires comprehensive medical history, travel history, clinical symptoms and microscopic examination results, and the identification of plasmodium-infected red blood cells plays a key role in the identification of malaria. Examination is used to determine the red blood cells infected by malaria parasites, but the exi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/47G01N21/64
CPCG01N21/64G01N33/56905G01N21/51G01N2021/6493G01N33/80G01N2333/445G01N21/49G01N2021/4707G01N2021/4711G01N2021/4726Y02A50/30G01N2015/0065G01N2015/0069G01N2015/0073G01N2015/008G01N33/5094G01N21/47G01N21/6486G01N33/5091
Inventor 叶波钱程祁欢
Owner SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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