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Material for promoting cell growth adhering to wall and preparation method of material

A technology of adherence and cells, which is applied in the field of materials that promote cell adherence growth and its preparation, can solve the problems affecting cell proliferation and growth, and the difficulty of separating downstream products of cultured cells, etc., to achieve radiation sterilization and adherence good effect

Inactive Publication Date: 2013-05-08
高敏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, soybean hydrolyzate contains other components besides protein, which affects the proliferation and growth of cells, and brings difficulties to the separation of downstream products of cultured cells.

Method used

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  • Material for promoting cell growth adhering to wall and preparation method of material
  • Material for promoting cell growth adhering to wall and preparation method of material
  • Material for promoting cell growth adhering to wall and preparation method of material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Take 0.68 μg / ml mussel mucin solution and add it to a 6-well culture plate, the amount added to each well is 1.43 mL, then the concentration of mussel mucin in each well is 0.1 μg / cm 2 . Dry the culture plate in an oven at 40°C for later use.

[0078] BEK-21 cells were cultured in MAP-coated plates and empty plates (control group without MAP coating), and the cell growth was observed at 2, 12 and 48 hours respectively. The medium was DMEM without adding serum. The result is as Figure 1a , 1b , 2a, 2b, 3a, 3b.

[0079] Compared with the control, the cells on the culture plate coated with mussel mucin had adhered and expanded after 2 hours, and the cells on the culture plate coated with mussel mucin had grown into a monolayer after 48 hours.

Embodiment 2

[0081] Take 0.5mg / ml mussel mucin solution and add it to a 6-well culture plate, the amount added to each well is 1.425mL, then the concentration of mussel mucin in each well is 75μg / cm 2 . Dry the plate in an oven at 55°C.

[0082] BHK-21 cells were cultured in MAP-coated plates and empty plates (control group without MAP coating), and the cell growth was observed at 2, 12 and 48 hours respectively. The medium was DMEM and 10% fetal bovine serum was added. . The result is as Figure 4a , 4b , 5a, 5b, 6a, 6b.

[0083] Compared with the control, the cells on the culture plate coated with mussel mucin had adhered and expanded after 2 hours, and the cells on the culture plate coated with mussel mucin had grown into a monolayer after 48 hours.

Embodiment 3

[0085] Take the mussel mucin solution and add it to a culture bottle with a bottom area of ​​25 square centimeters to make the mussel mucin density reach 0.1 μg / cm 2 . Dry the plate in an oven at 20°C.

[0086] The mesenchymal stem cells were cultured in MAP-coated plates and empty plates (control group without MAP coating), and the cell growth was observed at 2 and 12 hours, respectively. The medium was DMEM, supplemented with 10% fetal bovine serum. The result is as Figure 7a , 7b , 8a, 8b.

[0087] Compared with the control, in the culture plate coated with mussel mucin, the cells had adhered to the wall and extended after 2 hours, and the number of cells increased significantly after 12 hours.

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Abstract

The invention provides a material for promoting cell growth adhering to a wall and a preparation method of the material. The material for promoting cell growth adhering to wall comprises a solid medium used for cell culture and a mussel adhesive protein adhered to the solid medium; the preparation method of the material comprises the following steps: adding a mussel adhesive protein solution into a culture vessel or a suspension culture carrier and automatically adhering the mussel adhesive protein to the solid surface for use. The method and the material provided by the invention can promote the cell to fast adhere to the wall and crawl; and the material can be preserved at normal temperatures, can tolerate sterilization of gamma rays and reduce the usage amount of supernatant serum and is especially applied to the serum-free culture environment.

Description

technical field [0001] The invention relates to a cell culture material and a preparation method, in particular to a material for promoting cell adherent growth prepared by using mussel mucin as a main raw material and a preparation method thereof. Background technique [0002] Animal tissue culture plays an important role in the biotechnology industry due to the ability of animal cells to synthesize and secrete complex proteins. In animal cell culture, the growth of most cells is anchorage-dependent. Vessels for mammalian cell culture and analysis involving anchorage-dependent cells are typically constructed of glass or polymers, which often require additional surface treatments to allow cells to adhere to the vessel surface. Surface treatment methods generally include physical methods and chemical methods. Physical treatment is mainly achieved by means of the characteristics of the container surface, using radio frequency vacuum plasma, DC glow discharge and microwave pl...

Claims

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Application Information

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IPC IPC(8): C12N5/07C12N5/077C12N5/071
Inventor 高敏
Owner 高敏
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