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Culture medium for preparing influenza vaccine through MDCK cells and application method thereof

A technology of influenza vaccine and culture medium, which is applied in the field of culture medium for preparing influenza virus vaccine by cultivating MDCK cells, can solve the problems that the difference between product batches is difficult to control, the research needs to be further carried out, and the composition is difficult to be determined, so as to achieve good cell morphology and reduce risk, fast-growing effects

Active Publication Date: 2013-05-01
杭州国牧生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the disadvantages of animal-derived serum are: the composition is difficult to determine, the difference between product batches is not easy to control, the price is expensive, and extremely strict testing procedures are required to ensure that the serum does not contain bacteria, viruses, etc. that may be carried by the serum-derived animal
However, research on the medium for the preparation of influenza vaccines by large-scale high-density culture of MDCK cells in bioreactors needs to be further carried out

Method used

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  • Culture medium for preparing influenza vaccine through MDCK cells and application method thereof
  • Culture medium for preparing influenza vaccine through MDCK cells and application method thereof
  • Culture medium for preparing influenza vaccine through MDCK cells and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The culture medium of the large-scale high-density culture of the MDCK cell that uses, its composition and the ratio of parts by weight are as follows:

[0051]

[0052]

[0053]3% fetal calf serum was added to the above medium to prepare a medium for large-scale high-density culture of MDCK cells.

[0054] The composition of the medium used to maintain virus proliferation and the ratio of parts by weight are as follows:

[0055]

[0056]

[0057] On the basis of the above-mentioned medium, the following components are also added to make a culture solution for maintaining virus proliferation, and the ratio of its parts by weight is as follows:

[0058]

[0059]

[0060] The experimental results are shown in Table 1, the data of "Example 1" in Table 2.

Embodiment 2

[0062] The culture medium of the large-scale high-density culture of the MDCK cell that uses, its composition and the ratio of parts by weight are as follows:

[0063]

[0064]

[0065] 3% fetal calf serum was added to the above medium to prepare a medium for large-scale high-density culture of MDCK cells.

[0066] The composition of the medium used to maintain virus proliferation and the ratio of parts by weight are as follows:

[0067]

[0068]

[0069] On the basis of the above-mentioned medium, the following components are also added to make a culture solution for maintaining virus proliferation, and the ratio of its parts by weight is as follows:

[0070]

[0071] The experimental results are shown in Table 1 and the data of "Example 2" in Table 2.

Embodiment 3

[0073] The culture medium of the large-scale high-density culture of the MDCK cell that uses, its composition and the ratio of parts by weight are as follows:

[0074]

[0075]

[0076] 3% fetal calf serum was added to the above medium to prepare a medium for large-scale high-density culture of MDCK cells.

[0077] The composition of the medium used to maintain virus proliferation and the ratio of parts by weight are as follows:

[0078]

[0079]

[0080] On the basis of the above-mentioned medium, the following components are also added to make a culture solution for maintaining virus proliferation, and the ratio of its parts by weight is as follows:

[0081]

[0082] The experimental results are shown in Table 1, the data of "Example 3" in Table 2.

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Abstract

The invention provides a culture medium suitable for large-scale high-density cultivation of MDCK (Madin Darby Canine Kidney) cells for preparation of influenza vaccine.

Description

technical field [0001] The invention belongs to the field of biological products, and relates to a method for configuring and using a medium, and more specifically, relates to a medium for preparing influenza virus vaccines by culturing MDCK cells in a large-scale high-density growth environment. Background technique [0002] The production of final products through animal cell culture is one of the most commonly used production methods in the field of biological products. Most of the medium used for animal cell culture needs to add 10%-15% animal serum to provide the nutrients needed for cell growth and the supporting proteins for cells to maintain normal shape. However, the disadvantages of animal-derived serum are: the composition is difficult to determine, the difference between product batches is not easy to control, the price is expensive, and extremely strict testing procedures are required to ensure that the serum does not contain bacteria, viruses, etc. that may be ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/07C12N7/00A61K39/145A61P31/16
Inventor 贾馨丹
Owner 杭州国牧生物科技有限公司
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