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Acinetobacter calcoaceticus for promoting rhizosphere growth of walnuts and application thereof

A rhizosphere growth-promoting, calcium acetate technology, applied in the direction of application, bacteria, biocide, etc., can solve the problems of screening that have not been reported, and achieve the effects of promoting root growth, increasing the number of lateral roots, and excellent strain resources

Inactive Publication Date: 2013-04-24
SHANDONG FOREST SCI RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there is no report on the screening of walnut-specific PGPR

Method used

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  • Acinetobacter calcoaceticus for promoting rhizosphere growth of walnuts and application thereof
  • Acinetobacter calcoaceticus for promoting rhizosphere growth of walnuts and application thereof
  • Acinetobacter calcoaceticus for promoting rhizosphere growth of walnuts and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Example 1 Screening of walnut rhizosphere growth-promoting Acinetobacter calcium acetate

[0020] (1) Screening process:

[0021] 342 isolates were isolated from walnut rhizosphere soil samples in Caofan Town, Zhangqiu City, Shandong Province by gradient dilution method. Purify the isolated bacteria by the three-section line method, and judge whether the strain is purified by microscopic examination, number the purified bacteria, pick a single colony, and transfer it to an LB slant for future use. 21 strains with better green retention effect were screened out by the method of green preservation, combined with the radish cotyledon weight gain method, finally one strain of bacteria with good growth promotion effect was screened out, which was recorded as X128.

[0022] (2) Colony characteristics and cell morphology:

[0023] After X128 was cultured on LB plates for 24 hours, colonies formed were shiny, round and translucent. The surface is smooth, flat, and the edges ...

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Abstract

The invention discloses acinetobacter calcoaceticus for promoting rhizosphere growth of walnuts. The acinetobacter calcoaceticus is named acinetobacter calcoaceticus (Acinetobacter calcoaceticus) X128 in class, and preserved at the general microbiological center of the China Committee for Culture Collection of Microorganisms on December 31, 2012; and the preservation number is CGMCC No.7071. The invention also provides application of the acinetobacter calcoaceticus for promoting rhizosphere growth of the walnuts. The acinetobacter calcoaceticus for promoting rhizosphere growth of the walnuts is evenly fed to the periphery of the walnut root in a pouring manner; root growth can be obviously facilitated; the quantity of lateral roots is improved; and growth of the bottom diameter of the walnut and the height of seedling is facilitated. In contrast, the difference reaches a significant level. Therefore, excellent strain resources are provided for developing a special microbial fertilizer for the walnut in the future.

Description

technical field [0001] The invention relates to a walnut rhizosphere growth-promoting Acinetobacter calcium acetate and application thereof. Background technique [0002] Walnut (Juglans regia L) is a plant of the family Juglans and the genus Juglans. It ranks first among the four major nuts in the world and is known as the "king of woody oils". Walnut is also an important timber and ecological greening tree species. It is widely planted in mountainous areas, hills, rural areas and urban and rural areas, and has multiple economic, ecological and social benefits. China is the world's largest producer and consumer of walnuts, with walnut cultivation area and total nut production ranking first in the world, and walnuts are distributed in more than 20 provinces (autonomous regions and municipalities) across the country. According to statistics, in 2010, the total area of ​​walnut planting in China exceeded 2 million hectares, and the total annual nut production exceeded 900,000...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P21/00C12R1/01
Inventor 马海林邢尚军刘方春杜振宇马丙尧杜秉海丁延琴姚良同陈波
Owner SHANDONG FOREST SCI RES INST
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