A synbiotic feed additive, and a preparation method and applications thereof
A technology of feed additives and synbiotics, which is applied in the field of animal feed and feed additives, can solve problems that need to be improved, and achieve the effects of improving the balance of intestinal flora, improving the health of animals, and improving the immunity of livestock and poultry
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[0019] The method for preparing the synbiotic additive of the present invention generally comprises the following steps:
[0020] First, at least one of 1 to 5 parts by weight selected from chitosan, chitosan and seaweed oligosaccharide, 2 to 10 parts by weight selected from at least one of astragalus polysaccharide, wolfberry polysaccharide and hawthorn flavonoids, and 100 parts by weight of A probiotic preparation composition, wherein the probiotic preparation composition is composed of Bacillus subtilis, Bacillus coagulans and Clostridium butyricum with a weight ratio of 1-3:1-3:1-3.
[0021] Then, at least one of the above-mentioned selected from chitosan oligosaccharides, chitosan and seaweed oligosaccharides, at least one selected from astragalus polysaccharides, wolfberry polysaccharides and hawthorn flavonoids, and the probiotic composition are mixed uniformly, so as to obtain the synbiotic Vegetarian feed additive.
Embodiment 1
[0023] Materials and reagents: chitosan oligosaccharide (Dalian Zhongke Geleike Biotechnology Co., Ltd., MP103), astragalus polysaccharide (Xianyang Aviation 168 Bioengineering Company, HK-169), Bacillus coagulans (Skaida Biotechnology ( Kunshan) Co., Ltd. ningjie001), Clostridium butyricum strains (Sukehan (Weifang) Bioengineering Co., Ltd., SUKAFEED-B.Sub), Bacillus subtilis strains (purchased from China Industrial Microbiology Collection Center (Clostridium butyricum CICC20036)).
[0024] equipment:
[0025] 1. Seed tank (GYS-500C);
[0026] 2. Fermentation tank (GYS-1500C);
[0027] 3. Fermentation incubator (SPX-1500);
[0028] 4. Stirring and pulverizing machine (SWFL82D);
[0029] 5. Horizontal pressure steam sterilizer (ZY.01.001);
[0030] 6. High-speed centrifugal spray dryer (LPG1000);
[0031]7. Shrink packaging machine (SM-4525L);
[0032] 8. Quantitative powder filling machine (DXDCF2000A).
[0033] First, according to the solid fermentation method, live ...
Embodiment 2
[0051] With reference to the aforementioned general method, prepare the synbiotic feed additive of the present invention according to the following steps:
[0052] First, according to the weight ratio of 3:2:2, the live bacteria dry powder preparations of Bacillus subtilis, Bacillus coagulans and Clostridium butyricum prepared in Example 1 were mixed, so as to obtain a probiotics composition.
[0053] Then, 1 weight part of chitosan oligosaccharide, 2 weight parts of astragalus polysaccharide and 100 weight parts of the above-mentioned probiotic composition were mixed evenly, so as to obtain the synbiotic feed additive of the present invention.
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