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Method for controlling pests

A pest, protein technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, pesticides, etc.

Inactive Publication Date: 2013-04-17
BEIJING DABEINONG TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Plants transgenic for Vip3A gene have been proven to be resistant to Lepidoptera pests such as cutworm, fall armyworm, and corn armyworm, however, so far there is no research on producing transgenic plants expressing Vip3A protein to control the large borer Reports on Plant Hazards

Method used

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  • Method for controlling pests
  • Method for controlling pests
  • Method for controlling pests

Examples

Experimental program
Comparison scheme
Effect test

no. 1 example

[0084] The first embodiment, the acquisition and synthesis of Vip3A gene

[0085] 1. Obtain the nucleotide sequence of Vip3A

[0086] Amino acid sequence (789 amino acids) of Vip3Aa-01 insecticidal protein, as shown in SEQ ID NO: 1 in the sequence listing; Vip3Aa-01 encoding the amino acid sequence (789 amino acids) corresponding to said Vip3Aa-01 insecticidal protein Nucleotide sequence (2370 nucleotides), as shown in SEQ ID NO:3 in the sequence listing. Amino acid sequence (789 amino acids) of the Vip3Aa-02 insecticidal protein, as shown in SEQ ID NO: 2 in the sequence listing; Vip3Aa-02 encoding the amino acid sequence (789 amino acids) corresponding to the Vip3Aa-02 insecticidal protein Nucleotide sequence (2370 nucleotides), as shown in SEQ ID NO:4 in the sequence listing.

[0087] 2. Obtain Cry1A and Cry1F nucleotide sequences

[0088] The Cry1Ab nucleotide sequence (1848 nucleotides) encoding the amino acid sequence (615 amino acids) of the Cry1Ab insecticidal protei...

no. 2 example

[0091] The second embodiment, construction of recombinant expression vector and transformation of recombinant expression vector into Agrobacterium

[0092] 1. Construction of a recombinant cloning vector containing the Vip3A gene

[0093] The synthetic Vip3Aa-01 nucleotide sequence was ligated into the cloning vector pGEM-T (Promega, Madison, USA, CAT: A3600), and the operation steps were carried out according to the instructions of the pGEM-T vector produced by Promega Company to obtain the recombinant cloning vector DBN01-T , its construction process is as follows figure 1 Shown (wherein, Amp represents the ampicillin resistance gene; f1 represents the replication origin of phage f1; LacZ is the LacZ start codon; SP6 is the SP6 RNA polymerase promoter; T7 is the T7 RNA polymerase promoter; Vip3Aa-01 is the nucleotide sequence of Vip3Aa-01 (SEQ ID NO: 3); MCS is the multiple cloning site).

[0094]Then, the recombinant cloning vector DBN01-T was transformed into Escherichia...

no. 3 example

[0106] The third embodiment, the acquisition and verification of corn plants transferred to Vip3A gene

[0107] 1. Obtaining corn plants transferred to the Vip3A gene

[0108] According to the commonly used Agrobacterium infection method, the immature embryos of the aseptically cultured maize variety Zong 31 (Z31) were co-cultured with the Agrobacterium described in 3 in the second example, so that the T-DNA in recombinant expression vectors DBN100066, DBN100003 and DBN100276 (including the promoter sequence of corn Ubiquitin gene, Vip3Aa-01 nucleotide sequence, Vip3Aa-02 nucleotide sequence, Cry1Ab nucleotide sequence, Cry1Fa nucleotide sequence , PMI gene and Nos terminator sequence) were transferred into the maize genome, and the maize plants transferred to the Vip3Aa-01 nucleotide sequence, the maize plants transferred to the Vip3Aa-01-Cry1Ab nucleotide sequence and the Vip3Aa -02-Cry1Fa nucleotide sequence of maize plants; meanwhile wild-type maize plants are used as con...

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Abstract

The invention relates to a method for controlling pests, comprising contacting sesamia inferen pests with Vip3A protein. According to the invention, the sesamia inferen pests are controlled by the Vip3A protein which is generated in plant bodies and is capable of killing sesamia inferen; compared with an agricultural prevention and treatment method, a chemical prevention and treatment method and a biological prevention and treatment method, the method provided by the invention performs the whole growth period and the whole plant protection on the plants so as to prevent and treat the prejudice of the sesamia inferen pests; and in addition, the method also has the characteristics of no pollution, no residue, stable and complete effect, simplicity, convenience and economy.

Description

technical field [0001] The invention relates to a method for controlling pests, in particular to a method for using Vip3A protein expressed in plants to control the plant damage caused by the large borer. Background technique [0002] Sesamia inferens belongs to Lepidoptera Noctuidae. It is an omnivorous pest. In addition to corn, it also damages rice, sugarcane, sorghum and other gramineous crops. It is widely distributed in central and southeastern my country, especially in Shaanxi and Henan. Most of the rice areas to the south. The borer larvae bore into the stems of crops and cause damage to dead core seedlings or the whole plant. The boreholes are generally large, and a large amount of insect feces are discharged out of the stems. Occurs heavier than spring corn. [0003] Corn and sorghum are important food crops in China. The annual loss of food due to the large borer is huge, and even affects the living conditions of the local population. In order to prevent and con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N47/44A01P7/04A01N63/50
CPCA01N37/46C07K14/32C12N15/8286Y02A40/146A01N63/50
Inventor 韩超庞洁丁德荣康越景王利君梁燕龙刘雁华钱盈佳杨秋妹田康乐赵晓娜
Owner BEIJING DABEINONG TECH GRP CO LTD
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