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Lactoferrin sequences, compositions and methods of corneal wound treatment

A technology of lactoferrin and composition, applied in the direction of drug combination, peptide/protein composition, drug delivery, etc., can solve problems such as reduced vision, delayed corneal epithelium healing, and inability to maintain hydration in the eyes

Inactive Publication Date: 2013-04-10
BRIEN HOLDEN VISION INST (AU)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in some cases, such as chemical injury, the healing of the corneal epithelium is delayed, leaving the underlying stroma vulnerable to infection and ulceration
In addition, the eyes cannot maintain normal hydration, resulting in cloudiness that reduces vision

Method used

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  • Lactoferrin sequences, compositions and methods of corneal wound treatment
  • Lactoferrin sequences, compositions and methods of corneal wound treatment
  • Lactoferrin sequences, compositions and methods of corneal wound treatment

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Effect test

Embodiment Construction

[0117] Using an alkali-induced wound model, the inventors of the present invention identified the structure of lactoferrin that promotes wound healing of human corneal epithelium.

[0118] In summary, BLF leaflets were separated by proteolysis by restricted trypsin and purified using cation exchange and size exclusion chromatography. According to their serine protease activity using the benzamidine affinity column and their catalytic activity, the isoform of bovine lactoferrin (BLF) was isolated and synthesized by the serine protease substrate Z-Phe-Arg-7-amide The hydrolysis of -4-methyl-coumarin, quantifies those of BLF leaflets. These parts and BLF (iron-free, iron-bound, deglycosylated, exposed to zwitterionic detergents, chaotropic agent denaturation, reduced and alkylated, and lactoferrin B peptide (LFcin B)) The promotion of wound healing was estimated by incubating with a monolayer of human limbal epithelial cells (wound caused by a filter paper disc soaked in 0.1M sodiu...

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Abstract

The present invention relates to pharmaceutical compositions containing lactoferrin, or fragments of it, and their use in the treatment of wounds, particularly corneal wounds. The present invention also provides a pharmaceutical composition comprising an effective amount of a polypeptide or peptidomimetic consisting essentially of the C-lobe of lactoferrin, or functionally active fragments or variants thereof.

Description

Invention field [0001] The present invention relates to pharmaceutical compositions containing lactoferrin or fragments thereof, and their use in the treatment of wounds, especially corneal wounds. Background of the invention [0002] The cornea is the transparent front part of the eye, covering the pupil, iris, and anterior chamber. One of the important functions of the cornea is to maintain normal vision by refracting light to the lens and retina. The human cornea is composed of five layers, of which the corneal epithelium is the frontmost layer and forms the surface of the cornea. [0003] The epithelial layer is mainly cellular and consists of cells called keratinocytes. This layer acts as a physical barrier to prevent, for example, the penetration of microorganisms into deeper, more vulnerable structures. The matrix is ​​under the epithelium and is mainly composed of collagen. It also contains other cells called corneal cells, which play a role in stromal wound healing. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/40A61P27/02
CPCA61K38/40A61K9/0048A61P27/02A61K38/482
Inventor 本杰明·大卫·阿什比齐安·加勒特马克·威尔科克斯
Owner BRIEN HOLDEN VISION INST (AU)
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