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Quick mouth mucosa exudate detection method

A technology of oral mucosa and detection method, which is applied in the field of rapid detection of oral mucosa exudate, and can solve the problems of inconvenient test strips for occlusal detection

Inactive Publication Date: 2013-04-10
BEIJING MARR BIO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the assay via the above-mentioned test strips has the advantage of being straightforward, rapid and does not require complicated steps, it is inconvenient for the subject to bite the test strip throughout the test

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Assay strips suitable for use in the present invention may be obtained from any source including natural, chemically synthesized or recombinantly produced using methods known to those skilled in the art. For example, preferred peptide moieties of SEQ ID Nos. 1 to 5 can be chemically synthesized using solid phase peptide synthesis techniques, or by operatively linking a nucleic acid encoding the desired peptide into an expression vector and expressing the nucleic acid in a suitable host for recombinant production. Once isolated, the peptides can be biotinylated using known techniques.

[0032] Suitable antigens can be immobilized to the substrate using any method known to those skilled in the art that does not disrupt specific binding of the antigen to the target antibody.

[0033] For water-absorbing matrices, the streptavidin of the complex is typically coupled to the matrix prior to blocking, using techniques well known to those skilled in the art.

[0034] Preferab...

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PUM

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Abstract

The invention discloses a quick mouth mucosa exudate detection method and belongs to the field of biotechnology. The method comprises the following steps: lightly reversing the bottom for about three times to mix the sample buffer solution in the bottle; distributing the mouth mucosa exudate in a dropper into a test tube; taking a clean swab, immediately placing the swab into the tube with the sample buffer solution; repeatedly inserting the swab into the sample buffer solution tube for 6-8 times, and rubbing two sides of the swap by leaning against the tube wall; taking out the swap from the tube; and preparing the mouth mucosa exudate for detection; transferring 200 microliters of mouth mucosa exudate to an empty test tube; taking out a determination test strip from a jar, and immediately covering the jar; avoiding touching the membrane surface at the center of the test strip by a hand; placing the determination test strip into the tube with 200 microliters of diluted sample, making the arrow on the determination test strip downward; recording the time of the determination test strip added into the sample; reading the detection result after 20 minutes; and throwing the test strip, the tube and the swap into a biological dangerous cargo container.

Description

technical field [0001] The invention relates to a rapid detection method for oral mucosal exudate, which belongs to the field of biotechnology. Background technique [0002] Many analytical methods have been developed for the qualitative or quantitative detection of various analytes in biological tissues and fluids. For example, as a reagent for diagnosing oral diseases, Bengal Red is used as a staining agent, which has affinity with tumor cell DNA polymerase, and its coloring is specific to the diagnosis of oral cancer, and its coloring depth can distinguish oral cancer and precancerous damage, and prepares Bengal Red Oral Cancer Diagnostic Reagent is composed of the following raw materials: sodium bicarbonate, formaldehyde or ethanol, Bengal Red, and distilled water, all of which are prepared in certain proportions. [0003] A detection method for Streptococcus mutans and Streptococcus villosa in human saliva, the first step is to design primers, the provided nested polym...

Claims

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Application Information

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IPC IPC(8): G01N33/558
Inventor 刘满江
Owner BEIJING MARR BIO PHARMA
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