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Biological thermochemistry method for safety evaluation and screening of cigarette auxiliary material

A technology for safety evaluation and auxiliary materials, applied in the field of cigarette harm reduction effect and safety, which can solve the problems of long cycle, high cost and low efficiency.

Active Publication Date: 2013-03-20
HUBEI CHINA TOBACCO IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a biothermochemical method for the safety evaluation and screening of cigarette auxiliary materials in view of the defects of high cost, long cycle and low efficiency in the current auxiliary material in vitro toxicological detection method

Method used

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  • Biological thermochemistry method for safety evaluation and screening of cigarette auxiliary material
  • Biological thermochemistry method for safety evaluation and screening of cigarette auxiliary material
  • Biological thermochemistry method for safety evaluation and screening of cigarette auxiliary material

Examples

Experimental program
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Effect test

Embodiment 1

[0034] The preliminary identification of the biological effect of a new type of titanium dioxide-doped fullerene harm reduction material for cigarettes is designated as CB02. This new type of fullerene material has a very good purification effect on harmful components in flue gas. Escherichia coli, Staphylococcus aureus, and Candida albicans were used as models to study organisms, and their metabolic processes were monitored in real time by TAM3 microcalorimeter. We collected the mainstream smoke of the cigarette after adding this material, and compared it with the smoke of the same kind of cigarette without adding this material. Put the Cambridge filters that have captured mainstream smoke into numbered wide-mouth conical flasks, add a certain amount of 10ml of distilled water to soak and shake for 30min. After filtration, accurately take out 100 μl of the filtrate corresponding to the number from each Erlenmeyer flask and add it to 5 ml of culture medium that has been inocu...

Embodiment 2

[0038] The filter effects of four new functional carbon nanotube composite filters for cigarettes were analyzed. The first two are mostly walled carbon nanotubes, with diameters of 5 nm and 10 nm, respectively; the latter two are single-walled carbon nanotubes, with diameters of 2 nm and 1 nm, respectively. Theoretically, these four filters have different degrees of adsorption and selectivity effects on smoke, and they are numbered 1#, 2#, 3# and 4# respectively. Using microcalorimetry technology can quickly and accurately compare the filtering capabilities of these four filters and evaluate their filtering capabilities. Screening and excluding filters with obviously poor effects does not require evaluation of pilot tests or industrial applications, which saves a lot of money and time. The same brand of shredded tobacco was used in the experiment, and the cigarette of this brand was used as the reference cigarette. Saccharomyces cerevisiae, Lactobacillus plantarum, Bacillus ...

Embodiment 3

[0042] For improving the burning effect and slowing down the burning speed of the two new types of cigarette paper, they are numbered 1# and 2# respectively. These two kinds of cigarette papers were respectively added with 2 different flame retardants - 1# was added with aluminum hydroxide (1%), and 2# was added with magnesium hydroxide (1%). The two flame retardants can slow down the burning speed of the cigarette paper, make the burning process more complete, reduce the release of harmful substances, and are easy to prepare and low in cost. Before carrying out large-scale industrial testing, we first use microcalorimetry to conduct a preliminary assessment of the harm reduction effect. Taking Bacillus thuringiensis as a model to study organisms, TAM 3 microcalorimeter was used to monitor the influence of smoke components after burning two kinds of cigarette papers on Bacillus thuringiensis. The microcalorimetry experiment obtained various physical and chemical indicators re...

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Abstract

The invention discloses a biological thermochemistry method for safety evaluation and screening of cigarette auxiliary material. According to the method, an external macro micro-thermal experiment is adopted, the whole metabolic process of a microexamination object after disposed is monitored in real time through an isothermal thermal activity detector, and heat change in the gathering module collecting process is utilized to calculate relative physicochemical parameters. The method is easy in operation, extremely low in cost, short in experiment period, high in efficiency and reliable in result accuracy. The whole metabolic process can be observed in real time, a new method is created for evaluating cigarette harm reducing effects and screening additions and the method provides an important reference basis for further analysis evaluations in the following steps. The method not only promotes efficiency but also greatly reduces the cost.

Description

technical field [0001] The invention relates to the fields of harm reduction effect and safety of cigarettes, in particular to a biothermochemical method for safety evaluation and screening of auxiliary materials of cigarettes. Background technique [0002] In recent decades, food safety issues have received unprecedented attention. Although tobacco is not eaten directly in most cases, many countries still classify tobacco as food or quasi-food. As a "special food", its safety Sexuality has become more and more concerned, and testing procedures and methods have become more stringent. CORESTA (International Tobacco Scientific Research Collaboration Center) established a relevant tobacco smoke in vitro toxicity test working group, whose main task is to modify the internationally recognized in vitro toxicity test guidelines to adapt to the nature and unique characteristics of tobacco smoke, and at the same time Determine standard test methods. In vitro toxicity testing is not...

Claims

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Application Information

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IPC IPC(8): G01N25/48
Inventor 李冉宋旭艳魏敏罗诚浩陈义坤
Owner HUBEI CHINA TOBACCO IND
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